褐藻多糖硫酸酯调控肠道菌群减轻慢性肾病模型小鼠结肠炎症及肾脏损伤  

Fucoidan modulates gut microbiota alleviating colonic inflammation and renal injury in a mice of chronic kidney disease

作　　者：吕欣晨 张悦 王凉 陈宇滔 陆文伟[1] 王鸿超[1] LYU Xinchen;ZHANG Yue;WANG Liang;CHEN Yutao;LU Wenwei;WANG Hongchao(School of Food Science and Technology,Jiangnan University,Wuxi 214122,China;The Affiliated Wuxi People’s Hospital of Nanjing Medical University,Wuxi 214023,China)

机构地区：[1]江南大学食品学院,江苏无锡214122 [2]南京医科大学附属无锡人民医院,江苏无锡214023

出　　处：《食品与发酵工业》2025年第4期104-111,共8页Food and Fermentation Industries

基　　金：国家重点研发计划(2022YFF1100403)。

摘　　要：褐藻多糖硫酸酯(fucoidan,FPS)是一种从海藻中提取的硫酸化多糖,具有肾脏保护作用。该研究采用腺嘌呤诱导的慢性肾病小鼠,进行为期4周的FPS干预,旨在揭示FPS修复慢性肾病相关肠屏障损伤的作用,及其对肠道菌群组成和代谢功能的影响。结果表明,相比于模型组,FPS能够显著降低慢性肾病小鼠血清肌酐和尿素氮的水平,减轻肾纤维化程度。FPS对肠上皮紧密连接蛋白表达的改善效果有限,但能够显著降低结肠炎症。宏基因组结果显示,FPS调节了肠道菌群的组成和代谢功能,显著富集有益菌Akkermansia muciniphila、Bacteroides caecimuris,并抑制肠道致病菌Enterorhabdus caecimuris、Staphylococcus lentus的相对丰度。此外,FPS显著提升了慢性肾病小鼠肠道菌群氨基酸和维生素的生物合成能力,与慢性肾病的血管疾病相关。综上所述,该研究揭示了FPS通过调节特定肠道菌群及其代谢功能缓解结肠炎症,从而改善慢性肾病小鼠的肾脏病理。为深入研究FPS缓解肾损伤的作用机制提供新的见解。Fucoidan(FPS),a seaweed-derived polysaccharide,shows renal protective effects.The study conducted a four-week intervention with FPS on adenine-induced chronic kidney disease(CKD)mice,aiming to elucidate the role of fucoidan sulfated polysaccharide in repairing CKD-associated intestinal barrier damage and its impact on the composition and metabolic functions of gut microbiota.Results demonstrated that compared to the model group,FPS significantly reduced serum creatinine and blood urea nitrogen levels in CKD mice,alleviating renal fibrosis.While the improvement in tight junction protein expression in the intestinal epithelium was limited,FPS exhibited a notable reduction in colonic inflammation.Metagenomic sequencing revealed that FPS modulated the composition and metabolic functions of the gut microbiota,significantly enriching beneficial bacteria such as Akkermansia muciniphila and Bacteroides caecimuris,while inhibiting the relative abundance of pathogenic bacteria like Enterorhabdus caecimuris and Staphylococcus lentus.Additionally,FPS significantly enhanced the biosynthetic capabilities of amino acids and vitamins in the intestinal microbiota of CKD mice,particularly pathways related to vascular diseases in CKD.In conclusion,this study elucidates that FPS mitigates colonic inflammation by regulating specific gut microbiota and their metabolic functions,thereby improving renal pathology in CKD mice.This provides new insights for further investigating the mechanisms of action of FPS in the treatment of CKD.

关 键 词：褐藻多糖硫酸酯 慢性肾病 肠道屏障 肠道菌群 宏基因组 

分 类 号：TS201.4[轻工技术与工程—食品科学] R574.62[轻工技术与工程—食品科学与工程] R692[医药卫生—消化系统]