PD-L1 targeted iron oxide SERS bioprobe for accurately detecting circulating tumor cells and delineating tumor boundary  

作　　者：Ting Pan Dinghu Zhang Guomei You Xiaoxia Wu Chenguang Zhang Xinyu Miao Wenzhi Ren Yiwei He Lulu He Yuanchuan Gong Jie Lin Aiguo Wu Guoliang Shao 

机构地区：[1]Department of Interventional Radiology,Zhejiang Cancer Hospital,Institute of Basic Medicine and Cancer(IBMC),Chinese Academy of Sciences,Hangzhou 310022,China [2]Ningbo Key Laboratory of Biomedical Imaging Probe Materials and Technology,Cixi Institute of Biomedical Engineering,International Cooperation Base of Biomedical Materials Technology and Application,Chinese Academy of Science(CAS)Key Laboratory of Magnetic Materials and Devices and Zhejiang Engineering Research Center for Biomedical Materials,Ningbo Institute of Materials Technology and Engineering,CAS,Ningbo 315201,China [3]Advanced Energy Science and Technology Guangdong Laboratory,Huizhou 516000,China

出　　处：《Chinese Chemical Letters》2025年第1期408-414,共7页中国化学快报(英文版)

基　　金：supported by the funding from National Natural Science Foundation of China(Nos.32025021,12374390,31971292,82072032,82202274);Ningbo 3315 Innovative Teams Program(No.2019A-14-C);The member of Youth Innovation Promotion Association Foundation of CAS(No.2023310);Key Scientific and Technological Special Project of Ningbo City(Nos.2023Z209,2020Z189);National Key R&D Program of China(No.2019YFA0405603);Provincial Natural Science Foundation of Zhejiang(Nos.LQ23H180007,LQ23H180003);Zhejiang Province Science and Technology Plan of Traditional Chinese Medicine(No.2021KY085);Zhejiang Provincial Traditional Chinese Medicine Foundation(No.2021ZB04);the Major Medical and Health Science and Technology Project of Zhejiang Province(No.WKJ-ZJ-2002)。

摘　　要：Early diagnosis and accurate boundary delineation are the key steps of tumor precision medicine.Circulating tumor cells(CTCs)detection of liquid biopsy can provide abundant information for early diagnosis of cancer.High detection specificity and good enrichment features are two key factors for CTCs accurate identification in peripheral blood sample.For this purpose,iron oxide(IO)-based surface-enhanced Raman scattering(SERS)bioprobes with good biocompatibility,high detection sensitivity,remarkable detection specificity,and good enrichment efficiency,were developed for detecting different types of CTCs.Magnetic SERS bioprobes combined with programmed death ligand-1(PD-L1)antibody are regarded as an effective way to boost the targeting ability and detection specificity,benefiting for accurately capturing and identifying rare CTCs.Four types of CTCs with different PD-L1 expression were accurately distinguished among white blood cells via high-resolution SERS mapping images and stable Raman signals.Subsequently,CTCs blood samples obtained from the triple negative breast cancer patients were also successfully recognized compared to that of health people,indicating IO@AR@PDA-a PD-L1 SERS bioprobe possessed great potential for CTCs detection in liquid biopsy.Additionally,IO-based bioprobe exhibited excellent dual-modal imaging abilities of high-resolution SERS imaging mode and microimaging magnetic resonance imaging mode.These two highly complementary imaging modes endowed IO-based bioprobes unrivalled capacity in tumor boundary differentiation,supporting tumor accurate resection and precise surgery.To our best knowledge,this is the first time that biocompatible IO-based SERS bioprobes without noble metal element were reported not only for CTCs accurate detection,but also for precise tumor boundary delineation,showing great advantages in tumor diagnosis and treatment.

关 键 词：Circulating tumor cells Surface-enhanced Raman scattering PD-L1 Iron oxide bioprobe Dual-modal imaging Tumor boundaries delineation 

分 类 号：R73-3[医药卫生—肿瘤] O657.3[医药卫生—临床医学]