高效液相色谱法检测辣条中10种合成色素  

Determination of Ten Synthetic Colorants in Spicy Strips Based on High Performance Liquid Chromatography

作　　者：袁晓转 韩冰 张志超 王会宁 王颖莹 马骏 YUAN Xiaozhuan;HAN Bing;ZHANG Zhichao;WANG Huining;WANG Yingying;MA Jun(Inspection and Testing Center for Product Quality of Luoyang City,Luoyang 471000,China;Pharmacovigilance and Food Safety Monitoring Center of Luoyang City,Luoyang 471000,China;Administration for Market Regulation of Xixia County,Nanyang 474550,China)

机构地区：[1]洛阳市产品质量检验检测中心,河南洛阳471000 [2]洛阳市药物警戒和食品安全监测中心,河南洛阳471000 [3]西峡县市场监督管理局,河南南阳474550

出　　处：《食品科技》2024年第12期333-339,共7页Food Science and Technology

基　　金：河南省市场监督管理局科技计划项目(2022sj36)。

摘　　要：色素是辣条中添加较多的一类添加剂,存在超范围超限量添加的情况。文章建立了一种可同时测定辣条中10种常见人工合成色素(柠檬黄、金胺O、新红、苋菜红、胭脂红、日落黄、诱惑红、酸性红14、酸性橙7和赤藓红B)的高效液相色谱法。样品中加入15 mL水后用石油醚去除脂肪,经氨水乙醇溶液提取并氮吹浓缩后,用固相萃取小柱净化萃取,最后通过高效液相色谱仪进行分析。结果表明,10种色素在0.1~10μg/mL浓度范围内线性良好;方法检出限为0.3~0.5 mg/kg,定量限为1.0~1.5 mg/kg;加标回收率为71.35%~104.5%,精密度为0.81%~5.96%。该方法准确度高,检出限、定量限、回收率等指标均能满足要求,适用于辣条中10种人工合成色素的同时检测。Pigments are a commonly used class of additives in spicy strips,and there are cases of excessive and out-of-limit use.This study established a high-performance liquid chromatography(HPLC)method for the simultaneous determination of 10 common synthetic colorants(Lemon yellow,Auramine O,New red,Amaranth,Carmine,Sunset yellow,Tempting red,Acid red 14,Acid orange 7,Erythrosin B)in spicy strips.After adding 15 mL of water to the sample,fat was removed using petroleum ether,and the sample was extracted with ammonia-ethanol solution and concentrated by nitrogen blowing.Solid-phase extraction(SPE)columns were then used for purification,followed by HPLC analysis.The results showed that the 10 colorants exhibited good linearity within the concentration range of 0.1μg/mL to 10μg/mL,with detection limits ranging from 0.3 mg/kg to 0.5 mg/kg,quantification limits ranging from 1.0 mg/kg to 1.5 mg/kg,recovery rates ranging from 71.35%to 104.5%,and precision ranging from 0.81%to 5.96%.This method has high accuracy,with detection limits,quantification limits,and recovery rates all meeting the required standards,making it suitable for the simultaneous detection of 10 synthetic colorants in spicy strips.

关 键 词：辣条 合成色素 固相萃取 高效液相色谱 

分 类 号：TS207.3[轻工技术与工程—食品科学]