Polystyrene nanoplastic exposure actives ferroptosis by oxidative stress-induced lipid peroxidation in porcine oocytes during maturation  

作　　者：Yijing He Tianhang Yu Heran Li Qinfeng Sun Miaoyu Chen Yiyi Lin Jianjun Dai Weihan Wang Qiao Li Shiqiang Ju 

机构地区：[1]MOE Joint International Research Laboratory of Animal Health and Food Safety,College of Veterinary Medicine,Nanjing Agricultural University,Nanjing 210095,China [2]Key Laboratory of Livestock and Poultry Resources(Pig)Evaluation and Utilization,Ministry of Agriculture and Rural Affairs,Institute of Animal Husbandry and Veterinary Science,Shanghai Academy of Agricultural Sciences,Shanghai 201106,China

出　　处：《Journal of Animal Science and Biotechnology》2024年第6期2316-2332,共17页畜牧与生物技术杂志(英文版)

基　　金：supported by the National Natural Science Foundation of China(31972759 and 31572589);the Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD)。

摘　　要：Background Polystyrene nanoplastics(PS-NPs)are becoming increasingly prevalent in the environment with great advancements in plastic products,and their potential health hazard to animals has received much attention.Several studies have reported the toxicity of PS-NPs to various tissues and cells;however,there is a paucity of information about whether PS-NPs exposure can have toxic effects on mammalian oocytes,especially livestock.Herein,porcine oocytes were used as the model to investigate the potential effects of PS-NPs on mammalian oocytes.Results The findings showed that different concentrations of PS-NPs(0,25,50 and 100μg/m L)entering into porcine oocytes could induce mitochondrial stress,including a significant decrease in mitochondrial membrane potential(MMP),and the destruction of the balance of mitochondrial dynamic and micromorphology.Furthermore,there was a marked increase in reactive oxygen species(ROS),which led to oocyte lipid peroxidation(LPO).PS-NPs exposure induced abnormal intracellular iron overload,and subsequently increased the expression of transferrin receptor(TfRC),solute carrier family 7 member 11(SLC7a11),and acyl-CoA synthetase long-chain family member 4(ACSL4),which resulted in ferroptosis in oocytes.PS-NPs also indu ced oocyte maturation failure,cytoskeletal dysfunction and DNA damage.Cotreatment with 5μmol/L ferrostatin-1(Fer-1,an inhibitor of ferroptosis)alleviated the cellular toxicity associated with PS-NPs exposure during porcine oocyte maturation.Conclusions In conclusion,PS-NPs caused ferroptosis in porcine oocytes by increasing oxidative stress and altering lipid metabolism,leading to the failure of oocyte maturation.

关 键 词：Ferroptosis Lipid peroxidation MITOCHONDRIA Polystyrene nanoplastics Porcine oocyte ROS 

分 类 号：S859.8[农业科学—临床兽医学]