Effects of grain intervention on hypothalamic function and the metabolome of blood and milk in dairy cows  

作　　者：Limei Lin Kaizhen Guo Huiting Ma Jiyou Zhang Zheng Lai Weiyun Zhu Shengyong Mao 

机构地区：[1]Laboratory of Gastrointestinal Microbiology,Jiangsu Key Laboratory of Gastrointestinal Nutrition and Animal Health,National Center for International Research On Animal Gut Nutrition,College of Animal Science and Technology,Nanjing Agricultural University,Nanjing 210095,China [2]Ruminant Nutrition and Feed Engineering Technology Research Center,College of Animal Science and Technology,Nanjing Agricultural University,Nanjing 210095,China

出　　处：《Journal of Animal Science and Biotechnology》2024年第6期2543-2552,共10页畜牧与生物技术杂志(英文版)

基　　金：funded by Natural Science Foundation of China(32072755)。

摘　　要：Background The hypothalamus plays a crucial role in the health and productivity of dairy cows,yet studies on its fun ctionality and its impact on peripheral circulation in these animals are relatively scarce,particularly regarding dietary interventions.Therefore,our study undertook a comprehensive analysis,incorporating both metabolomics and transcriptomics,to explore the effects of a grain-based diet on the functionality of the hypothalamus,as well as on blood and milk in dairy cows.Results The hypothalamic metabolome analysis revealed a significant reduction in prostaglandin E_(2)(PGE_(2))level as a prominent response to the grain-based diet introduction.Furthermore,the hypothalamic transcriptome profiling showed a nota ble upregulation in amino acid metabolism due to the grain-based diet.Conversely,the grain-based diet led to the downregulation of genes involved in the metabolic pathway from lecithin to PGE_(2),including phospholipase A2(PLA2G4E,PLA2G2A,and PLA2G12B),cyclooxygenase-2(COX2),and prostaglandin E synthase(PTGES).Additionally,the plasma metabolome analysis indicated a substantial decrease in the level of PGE_(2),along with a decline in adrenal steroid hormones(tetrahydrocortisol and pregnenolone)following the grain-based diet introduction.Analysis of the milk metabolome showed that the grain-based diet significantly increased uric acid level while notably decreasing PGE_(2)level.Importantly,PGE_(2)was identified as a critical metabolic marker in the hypothalamus,blood,and milk in response to grain intervention.Correlation analysis demonstrated a significant correlation among metabolic alterations in the hypothalamus,blood,and milk following the grain-based diet.Conclusions Our findings suggest a potential link between hypothalamic changes and alterations in peripheral circulation resulting from the introduction of a grain-based diet.

关 键 词：BLOOD Grain-based diet HYPOTHALAMUS Metabolomics MILK Prostaglandin E_(2) TRANSCRIPTOMICS 

分 类 号：S823[农业科学—畜牧学]