产蛋白酶高温菌株筛选及豆粕固态发酵条件优化  

Thermophilic Protease-producing Bacteria and Optimization of Solid-state Fermentation Conditions of Soybean Meal

作　　者：张军燕[1] 张焕新[1] 吴平 刘萍[1] 黄延莉 代春华[2] 何荣海[2] Zhang Junyan;Zhang Huanxin;Wu Ping;Liu Ping;Huang Yanli;Dai Chunhua;He Ronghai(Jiangsu Agriculture and Animal Husbandry Science and Technology Vocational College,Taizhou 225300;School of Food and Biology(Institute of Food Physical Processing),Jiangsu University,Zhenjiang 212013)

机构地区：[1]江苏农牧科技职业学院,泰州225300 [2]江苏大学食品与生物工程学院,镇江212013

出　　处：《中国粮油学报》2025年第1期84-89,共6页Journal of the Chinese Cereals and Oils Association

基　　金：江苏农牧科技职业学院校级科研项目(NSF2022ZR01),江苏农牧科技职业学院科技创新团队资助项目(NSF2023TC03),泰州市科技项目(TN202220),泰州市“311工程”第一层次培养对象科研资助项目(RCPY202101)。

摘　　要：从云烟卷烟中分离纯化后得到1株产蛋白酶能力较强的高温菌株YYC4。经16S rRNA基因序列分子生物学鉴定该菌株为地衣芽孢杆菌(Bacillus licheniformis)。通过单因素和响应面优化确定YYC4菌株高温固态发酵未灭菌豆粕的最佳条件为:料液比1∶1.80 g/mL、发酵温度55℃、接种量10^(7)CFU/g含水基质、相对湿度80%、发酵时间50 h、MgSO_(4)质量分数0.12%。在此条件下发酵,豆粕可溶性蛋白质量分数由4.92%升高至18.57%,总蛋白质量分数由40.42%增加至47.95%,胰蛋白酶抑制剂质量分数则由8.19 mg/kg降低至3.19 mg/kg。A thermophilic strain YYC4 with relatively strong ability of protease production was isolated from Yunyan cigarette.The strain was identified as Bacillus licheniformis by 16S rRNA gene phylogenetic and phylogenetic analysis.Combination of single factor and response surface optimization experiments was applied to optimize fermentation conditions of unsterilized soybean meal.After fermentation under the optimal conditions of solid-liquid ratio of 1∶1.80 g/mL,fermentation temperature of 55℃,inoculation of 10^(7)CFU/g water-containing substrate,relative humidity of 80%,incubation of 50 h and MgSO_(4)of 0.12%(m/m),the contents of soluble and crude protein in soybean meal were increased from 4.92%to 18.57%and from 40.42%to 47.95%,while trypsin inhibitor activity decreased from 8.19 mg/kg to 3.19 mg/kg,respectively.

关 键 词：豆粕 高温固态发酵 菌株筛选 发酵条件优化 

分 类 号：TS229[轻工技术与工程—粮食、油脂及植物蛋白工程]