核桃青皮多酚调控对哈密瓜采后活性氧代谢水平及腐烂率的影响  

Effects of regulation of walnut green peel polyphenols on postharvest active oxygen metabolism and reduction of rotten in Hami melon

作　　者：李慧 毕莹 王新宇 雷雅馨 张琪 黄帅 热扎·库忘德克 王静 LI Hui;BI Ying;WANG Xinyu;LEI Yaxin;ZHANG Qi;HUANG Shuai;Rezha kuwangdeke;WANG Jing(College of Food Sciences and Pharmacy,Xinjiang Agricultural University,Urumqi 830052,China)

机构地区：[1]新疆农业大学食品科学与药学学院,乌鲁木齐830052

出　　处：《新疆农业科学》2024年第12期2966-2975,共10页Xinjiang Agricultural Sciences

基　　金：中央林业财政林草科技推广示范项目(新[2022]TG11号);国家自然科学基金地区基金项目(32260613);新疆维吾尔自治区自然科学基金面上项目(2022D01A80)。

摘　　要：【目的】研究核桃青皮多酚对哈密瓜果实贮藏期间活性氧代谢(ROS)和腐烂率的影响。【方法】以西州密25号哈密瓜为试材,以蒸馏水为对照,采用30 mg/L核桃青皮多酚(前期试验所得)处理哈密瓜果实,2组处理均加入0.5 mL/L吐温-20,浸泡20 min,常温(温度(22±2)℃,相对湿度(20±2)%)贮藏18 d。每3 d测定1次哈密瓜果实腐烂指数、腐烂率、细胞膜透性、丙二醛(MDA)含量、超氧阴离子(O^(-)_(2))产生速率、过氧化氢含量(H_(2)O_(2))和活性氧代谢相关酶活性。【结果】30 mg/L核桃青皮多酚处理诱导哈密瓜果实超氧化物歧化酶(SOD)(除第6 d)、过氧化氢酶(CAT)(除第3 d)、抗坏血酸过氧化物酶(APX)(除第6 d)、谷胱甘肽还原酶(GR)等酶的活性增强,有效增强还原型谷胱甘肽(GSH)含量,降低H_(2)O_(2)含量和O^(-)_(2)生成速率,延缓细胞膜透性和丙二醛(MDA)含量的上升,显著降低哈密瓜果实腐烂指数和腐烂率(P<0.05),减轻哈密瓜果实的腐烂症状。【结论】核桃青皮多酚可通过调控哈密瓜采后活性氧代谢水平,延缓果实衰老和降低腐烂率。【Objective】To study the effect of walnut green peel polyphenols on reactive oxygen species metabolism(ROS)and decay of Hami melon fruit during storage.【Methods】30 mg/L walnut green skin polyphenols(obtained from preliminary experiments)were used to treat Hami melon fruit.0.5 mL/L Tween-20 was added to both treatments,soaked for 20 minutes,and stored at room temperature(temperature:(22±2)℃,relative humidity:(20±2)%)for 18 days to measure the decay index,decay rate,cell membrane permeability,malondialdehyde(MDA)content,superoxide anion(O^(-)_(2))production rate,hydrogen peroxide content(H_(2)O_(2)),and enzyme activity related to reactive oxygen metabolism of Hami melon fruit every 3 days.【Results】Superoxide dismutase(SOD)(except 6 d),catalase(except 3 d),ascorbic acid peroxidase(APX)(except 6 d)and glutathione reductase(GR)were enhanced by 30 mg/L walnut green skin polyphenol treatment.Meanwhile,glutathione(GSH)content was increased,H_(2)O_(2)content and O^(-)_(2)production rate were decreased,and the increase of cell membrane permeability and malondialdehyde(MDA)content could be effectively delayed,the decay index and decay rate of hami melon(P<0.05)were significantly reduced,and the decay symptom of Hami melon fruit was alleviated.【Conclusion】Walnut green skin polyphenols have a regulatory effect on reactive oxygen species metabolism during post harvest storage,thereby achieving the goal of delaying fruit aging and decay after harvest.

关 键 词：哈密瓜 核桃青皮多酚 采后 活性氧代谢 腐烂率 

分 类 号：S662.1[农业科学—果树学]