液相色谱-三重四极杆串联质谱法检测速冻调理肉制品中12种着色剂  

Determination of 12 colorants in Quick Frozen Prepared Meat Products by Liquid Chromatography Triple Quadrupole Tandem Mass Spectrometry

作　　者：姜薇 高金芳 耿苗苗 孙伟 张凤霞 刘翔宇 JIANG Wei;GAO Jinfang;GENG Miaomiao;SUN Wei;ZHANG Fengxia;LIU Xiangyu(Weifang Product Quality Inspection Research Institute,Weifang 261031,China)

机构地区：[1]潍坊市产品质量检验研究院,山东潍坊261031

出　　处：《食品安全导刊》2025年第4期85-89,共5页China Food Safety Magazine

基　　金：项目来源潍坊市科技发展计划(软科学)项目“预制菜产品质量安全与检测技术支撑的研究——以速冻调理肉制品中的着色剂为例”(2023RKX008)。

摘　　要：基于监管需求,借助于固相萃取技术,建立一种速冻调理肉制品中12种着色剂的液相色谱-三重四极杆质谱联用检测方法。在试样中加入乙醇-乙腈-氨水混合溶液进行提取,经SPE萃取柱进行净化,采取ChromCore C18色谱柱进行分离,外标法定量。结果显示,酸性橙Ⅱ的定量限为0.0025 mg·kg^(-1),其他11种着色剂的定量限均为0.25 mg·kg^(-1);12种着色剂保留时间重复性良好,相对标准偏差在0.21%~1.05%,且回收率在85.66%~104.86%,精密度在1.36%~5.74%。该方法操作简便,分析时间较短,为监检工作提供了技术支撑。To meet regulatory demands and utilizing SPE technology,a liquid chromatography-triple quadrupole mass spectrometry method was developed for the simultaneous detection of 12 colorants in frozen prepared meat products.Ethanol-acetonitrile-ammonia water mixture extraction solution was added to the sample,which was then purified using a solid-phase extraction column and separated on a ChromCore C18 chromatographic column.Quantification was achieved using the external standard method.The results indicated that the quantification limit for Acid Orange Ⅱ was 0.0025 mg·kg^(-1),while for the other 11 colorants,it was 0.25 mg·kg^(-1).The retention times for the 12 colorants exhibited good reproducibility,the relative standard deviation was 0.21%to 1.05%.The recovery rates ranged from 85.66%to 104.86%,with a precision range of 1.36%to 5.74%.This method is straightforward to operate and boasts a brief analysis time,thereby offering technical support for regulatory inspections.

关 键 词：固相萃取柱 调理肉制品 着色剂 

分 类 号：TS2[轻工技术与工程—食品科学与工程]