基于网络药理学探讨“玉米须-桑叶”药对干预糖尿病的作用机制  

Investigation on the function mechanism of"stigma maydis-mulberry leaf"drug pair in intervening diabetes based on network pharmacology

作　　者：许春 郑兰平 XU Chun;ZHENG Lanping(Honghe Hani and Yi Autonomous Prefecture Research Institute of Inspection and Testing,Yunnan,Mengzi 661199,China;College of Chinese Materia Medica,Yunnan University of Chinese Medicine,Yunnan,Kunming 650500,China)

机构地区：[1]云南省红河哈尼族彝族自治州检验检测院,云南蒙自661199 [2]云南中医药大学中药学院,云南昆明650500

出　　处：《中国医药科学》2025年第1期48-52,共5页China Medicine And Pharmacy

基　　金：云南省科技厅科技计划项目(202101AZ070001-056)。

摘　　要：目的通过网络药理学和分子对接技术探究“玉米须-桑叶”药对干预糖尿病的重要靶标。方法截至2024年4月通过中药系统药理学数据库检索“玉米须-桑叶”药对活性成分及靶点;通过GeneCard数据库检索糖尿病靶点;构建韦恩图获得交集靶点。在Cytoscape 3.9.1软件中构建“玉米须-桑叶”药对-活性成分-靶点网络图;构建蛋白质互作网络筛选核心靶点,借助David数据库对交集靶点进行基因本体富集和京都基因与基因组百科全书通路分析,通过CB-Docks软件进行分子对接。结果得到“玉米须-桑叶”药对有效成分40个,靶点1457个,糖尿病相关靶点4062个,交集靶点620个,核心靶点5个。分子对接分析发现红厚壳(种)内酯与磷脂酰肌醇-3-激酶催化亚基α(PIK3CA)结合能力最强。结论“玉米须-桑叶”药对可能通过热休克蛋白90α家族A类成员1、酪氨酸蛋白激酶、丝氨酸/苏氨酸蛋白激酶1、PIK3CA、雌激素受体α等靶标调控表皮生长因子受体酪氨酸激酶抑制剂耐药性、内分泌的阻力、磷酸化磷脂酰肌醇3-激酶等信号通路多因素共同调控干预糖尿病。Objective To investigate the important target of"stigma maydis-mulberry leaf"drug pair in intervening diabetes based on network pharmacology and molecular docking technology.Methods By April 2024,the active components and targets of"stigma maydis-mulberry leaf"drug pair were searched by Traditional Chinese Medicine Systematic Pharmacology Database.Diabetes target points were searched through GeneCard database.Wayne diagram was constructed to obtain intersection target.The network diagram of"stigma maydis-mulberry leaf"drug pair-active ingredient-target was constructed in Cytoscape 3.9.1 software.Protein-protein interaction network was constructed to screen the core targets.With the help of David database,gene ontology enrichment and Kyoto Encyclopedia of Genes and Genomes pathway analysis were carried out on the intersecting targets,and molecular docking was carried out by CB-Docks software.Results There were 40 active ingredients,1457 targets,4062 diabetes related targets,620 intersection targets and 5 core targets in the"stigma maydis-mulberry leaf"drug pair.Molecular docking analysis showed that the inophyllolide had the strongest binding ability with phosphatidylinositol-3-kinase catalytic subunitα(PIK3CA).Conclusion The"stigma maydis-mulberry leaf"drug pair can jointly regulate and intervene in diabetes through multiple factors,such as the regulation of epidermal growth factor receptor tyrosine kinase inhibitor resistance,endocrine resistance,phosphorylated phosphatidylinositol 3-kinase and other signal pathways,possibly through targets such as heat shock protein 90αfamily A member 1,tyrosine protein kinase,serine/threonine protein kinase 1,PIK3CA,estrogen receptorα.

关 键 词：玉米须 桑叶 网络药理学 糖尿病 分子对接 

分 类 号：R285[医药卫生—中药学]