甘露聚糖底物对干酪乳杆菌HDS-01生长及产甘露聚糖酶的影响  

Effects of mannans substrates on the growth and mannanase production of Lactobacillus casei HDS-01

作　　者：石景军 那金[1,2] 杨若兮 赵丹 SHI Jingjun;NA Jin;YANG Ruoxi;ZHAO Dan(Heilongjiang University a.Key Laboratory of Microbiology,Life Science College,Harbin 150080,China;Engineering Research Center of Agricultural Microbiology Technology,Ministry of Education,Harbin 150500,China)

机构地区：[1]黑龙江大学生命科学学院微生物省高校重点实验室,哈尔滨150080 [2]黑龙江大学农业微生物技术教育部工程研究中心,哈尔滨150080

出　　处：《黑龙江大学工程学报(中英俄文)》2024年第3期96-103,共8页Journal of Engineering of Heilongjiang University

基　　金：国家自然科学基金项目(32072189,32071519);广西多糖材料与改性重点实验室开放基金项目(GXPSMM20-2)

摘　　要：甘露聚糖酶在纺织、医疗、食品等工业领域备受关注,微生物是甘露聚糖酶生产的主要来源。乳酸菌(Lactic Acid Bacteria, LAB)甘露聚糖酶水解β-1,4-糖苷键连接骨架的甘露聚糖制备产生的甘露低聚糖(Mannan Oligosaccharides, MOS),是生物安全性高的潜在益生元。作为诱导物的甘露聚糖底物,是影响乳酸菌产酶的最重要因素。为了提高乳酸菌产酶水平,评估3种甘露聚糖底物魔芋粉(Konjac Glucomannan, KGM)、角豆胶(Locust Bean Gum, LBG)和瓜尔胶(Guar Gum, GUAR)对干酪乳杆菌(Lactobacillus casei)HDS-01生长、产酶及所得低聚糖的影响。当L.casei HDS-01处于含有0.2%葡萄糖的KGM培养基的发酵条件下,生长及产甘露聚糖酶最好,生物量最大为9.41 Log(CFU·mL^(-1)),酶活达到33.16±0.92 U·mL^(-1)。通过高效液相色谱法(High Pressure Liquid Chromatography, HPLC)检测甘露二糖、甘露三糖和甘露四糖3种低聚糖的出峰面积计算产物浓度,最大分别为0.63±0.005、0.32±0.020和0.26±0.019 mg·mL^(-1)。为L.casei HDS-01甘露聚糖酶的扩大生产提供了依据,也为甘露低聚糖制备奠定了基础。Mannanase derived mainly from microorganisms has been widely used in various industrial fields such as textile,medicine and food processing.Mannanase produced by lactic acid bacteria(LAB)can hydrolyze theβ-1,4-glycosidic bonds in mannans to prepare mannan oligosaccharides(MOS)which is potential prebiotics with high bio-safety.In order to enhance the LAB mannanase-producing level,this study investigated the effects of three mannans substrates,i.e.Konjac Glucomannan(KGM),locust bean gum(LBG)and Guar gum(GUAR)on growth,enzyme production and MOS preparation of Lactobacillus casei HDS-01.When L.casei HDS-01 was cultured in the KGM media containing 0.2%glucose,the best fermentation results was obtained.The highest biomass reached 9.41 Log(CFU·mL^(-1))and the mannanase activity was 33.16±0.92 U·mL^(-1).The High Pressure Liquid Chromatography(HPLC)was used to determine the MOS concentration according peak area.The maximum concentration of mannobiose,mannotriose and mannotetraose was 0.63±0.005,0.32±0.020 and 0.26±0.019 mg·mL^(-1),respectively.The approach set a basis for the large-scale mannanase production by L.casei HDS-01 and also laid a foundation of MOS preparation.

关 键 词：甘露聚糖 甘露聚糖酶 干酪乳杆菌 魔芋粉 甘露低聚糖 

分 类 号：Q935[生物学—微生物学]