SPE-HPLC法测定食品中3种红曲色素  

Determination of three monascus pigments in food by SPE-HPLC

作　　者：吕卓璇 张玉 李营 LYU Zhuoxuan;ZHANG Yu;LI Ying(Xuzhou Center for Disease Control and Prevention,Xuzhou 221000,Jiangsu,China)

机构地区：[1]徐州市疾病预防控制中心,江苏徐州221000

出　　处：《中国校医》2024年第11期872-876,共5页Chinese Journal of School Doctor

摘　　要：目的 采用固相萃取-高效液相色谱法(SPE-HPLC法)对徐州地区市售食品中红曲红素、红曲素、红曲红胺3种红曲色素进行检测。方法 对比HLB和C18固相萃取柱,离心和过滤膜2种净化方式对样品前处理方法进行优化,调整流动相比例和梯度洗脱以及不同的检测波长对色谱条件进行优化,使用优化后的方法对市售熟肉制品、腌制蔬菜、腐乳、果酱、果冻中的3种红曲色素进行检测。结果 使用优化后的SPE-HPLC法,共检测26份样品,3种红曲色素分离度好,标准曲线线性关系良好,相关系数均>0.999 5,回收率为83.0%~86.1%,其中红曲素、红曲红素经HLB固相萃取柱和C18固相萃取柱测得的平均值差异有统计学意义(t=16.013、7.643,P均<0.001),经HLB固相萃取柱和C18固相萃取柱测得的红曲红胺平均值差异无统计学意义(t=0.665,P=0.521)。使用本方法对5类食品中的3种红曲色素进行检测,腐乳和熟肉制品中红曲红素检出率分别为83.3%和25.0%,红曲红胺和红曲素均未检出。结论优化后SPE-HPLC法,快速简便,准确度高,重现性好,可满足食品中红曲色素的检测要求。使用该方法检测26份市售食品,所有样品中红曲红胺和红曲素均未检出,红曲红素检出率最高。Objective Solid-phase extraction-high-performance liquid chromatography(SPE-HPLC)was used to determine monascus pigments and monascus red amine in food sold in Xuzhou.Methods HLB and C18 solid phase extraction columns,centrifugation,and filtration membranes were compared to optimize the sample pretreatment method.Chromatographic conditions were optimized by adjustment of the mobile phase ratio,gradient elution,and detection wavelengths.The optimized method was used for determination of three monascus pigments in commercial cooked meat products,pickled vegetables,preserved bean curd,jam and jelly.Results A total of 26 samples were analyzed with the optimized SPE-HPLC method.The standard curve showed a good linear relationship for the three monascus pigments,with correlation coefficients all exceeding 0.9995.The recovery rates ranged from 83.0%to 86.1%.Statistical differences were observed in the mean values of monastin and monasubin measured with an HLB solid phase extraction column and C18 solid phase extraction column(t=16.013,7.643,both P<0.001).However,no statistical difference was observed in the mean values of monasubin measured with an HLB solid phase extraction column and C18 solid phase extraction column(t=0.665,P=0.521).When this method was applied to detect three types of monascus pigments in five food categories,the detection rates of monascorubrin in fermented bean curd and processed meat products were 83.3%and 25.0%respectively,whereas monascus red amine and monascine were not detected.Conclusions Our optimized SPE-HPLC method was found to be simple,accurate,and reproducible,and met the requirements for detection of monascus pigments in food.Among the 26 commercially available food samples tested,no monascus red amine and monascus were detected,and the detection rate of monacolins was the highest.

关 键 词：市售食品 SPE-HPLC法 红曲色素 测定 

分 类 号：S207.3[农业科学—农业工程] O657.63[理学—分析化学]