基于PCR技术的高产木糖醇工程菌重组质粒的构建和鉴定  

Construction and identification of high-yield xylitol engineering bacterial recombinant plasmids based on PCR technology

作　　者：肖宏伟 叶有员 刘欣 XIAO Hongwei;YE Youyuan;LIU Xin(Beijing High School,Beijing 100018,China;Beijing Chaoyang District Institute of Education Science,Beijing 100021,China)

机构地区：[1]北京中学,北京100018 [2]北京市朝阳区教育科学研究院,北京100021

出　　处：《生物学教学》2025年第2期53-58,共6页Biology Teaching

摘　　要：通过“构建高产木糖醇工程菌”这一实际问题的研究,基于PCR实验原理,引导学生创新性地解决研究中的问题:在引物末端设计添加所需限制酶切位点,实现同源序列的扩增和阳性菌落的鉴定。在此过程中提升学生关键能力和生物学学科核心素养。PCR技术在基因工程实验教学中的应用,为高中生物实验教学提供新的思路和方法。Through the study of the practical issue of“constructing high-yield xylitol engineering bacteria,”it utilized the principles of PCR experiments to guide students in innovatively solving problems encountered in the research.This involved designing and adding the required restriction enzyme cutting sites at the ends of primers to achieve the amplification of homologous sequences and the identification of positive colonies.This process enhanced students key abilities and core competencies in the field of biology.The application of PCR technology in gene engineering experimental teaching provides new ideas and methods for high school biology laboratory education.

关 键 词：PCR技术 质粒构建 工程菌 高产木糖醇 高中生物学 

分 类 号：G63[文化科学—教育学]