茶饮料中茶多酚含量测定方法的研究  

Research on the Method for Determining Tea Polyphenol Content in Tea Beverages

作　　者：高树财 闫格 胡桂芳 高火亮 GAO Shu-cai;YAN Ge;HU Gui-fang;GAO Huo-liang(Henan Food Quality and Safety Control Engineering Technology Research Center,Henan Commerce Science Institute Co.,Ltd..)

机构地区：[1]河南省食品质量安全控制工程技术研究中心,河南省商业科学研究所有限责任公司

出　　处：《标准科学》2025年第1期99-103,共5页Standard Science

基　　金：河南省科学院食品安全与营养健康创新团队项目(项目编号:20230104)研究成果。

摘　　要：【目的】建立一种福林酚衍生比色法测定茶饮料中茶多酚含量的分析方法。【方法】采用单因素试验确定衍生的最佳条件,在此条件下绘制没食子酸标准系列溶液吸光度与浓度的标准曲线,并测定实际样品,同时与国标方法测得的结果进行比对。【结果】确定了在10mL溶液体系中,对标准系列溶液的最佳衍生条件为:福林酚用量1.0mol,碳酸钠用量1.5 mL,可在自然光线下进行,反应时长60min。所得标准曲线为y=0.100876x+0.015891,R^(2)=0.9989,线性范围100~2000 mg/kg,方法回收率为106.9%,相对标准偏差为0.6%(n=6)。【结论】与现行国标方法相比,该法适用浓度范围更广,基质干扰和仪器系统误差更小,准确度和精密度也更高,可为国标方法的改进提供参考。[Objective]The research aims to establish a Folin-Phenol derivative colorimetric analytical method for determining the content of tea polyphenols in tea beverage.[Method]The optimal conditions for derivatization are determined by one-factor experiments.Under those conditions,the standard curve of gallic acid is plotted by measuring absorbance and concentration of the standard series solution,and then the practical samples are measured.The results are compared with those measured by the national standard method.[Result]The optimal derivatization conditions for the standard series solution in a 10 mL system are determined as follows:Folin-phenol reagent usage of 1.0 mol,sodium carbonate usage of 1.5 mL,and the reaction can be carried out under natural light for 60 min.The standard curve is y=0.100876x+0.015891,R^(2)=0.9989,with a linear range of 100~2000 mg/kg,a method recovery rate of 106.9%,and a relative standard deviation of 0.6%(n=6).[Conclusion]Compared to the national standard method,the method has a wider applicable range of concentration,smaller matrix interference and instrumental system errors,and higher accuracy and precision,which can provide reference for improving the national standard method.

关 键 词：茶饮料 茶多酚 福林酚衍生比色法 

分 类 号：TS2[轻工技术与工程—食品科学与工程]