Integrated analysis of colorectal cancer metastasis identifies characteristics of tumor cell during metastasis  

作　　者：Haoyu Fu Xiaohuan Lu Tiantian Ji Liping Wang Guobin Wang Lin Wang Zheng Wang 

机构地区：[1]Department of Gastrointestinal Surgery,Union Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan,Hubei,P.R.China [2]Research Center for Tissue Engineering and Regenerative Medicine,Union Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan,Hubei,P.R.China [3]Hubei Key Laboratory of Regenerative Medicine and Multi-disciplinary Translational Research,Wuhan,Hubei,P.R.China [4]Hubei Provincial Engineering Research Center of Clinical Laboratory and Active Health Smart Equipment,Wuhan,Hubei,P.R.China [5]Department of Clinical Laboratory,Union Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan,Hubei,P.R.China

出　　处：《Gastroenterology Report》2024年第1期258-267,共10页胃肠病学报道(英文)

基　　金：supported by the National Natural Science Foundation of China Programs(81974382,82072068,82173315,82072167 and 82272277);the Technical Innovation Major Foundation Program of Hubei Province(2022BCA013);the National Key R&D Program of China(2022YFC2408100);Hubei Province Science and Technology Innovation Team Project([2022]No.11).

摘　　要：Background:Metastasis is the main cause of death in colorectal cancer(CRC).Metastasis is a sequential and dynamic process,but the development of tumor cells during this process is unclear.In this study,we aimed to reveal characteristics of tumor cell subset during CRC metastasis.Methods:Single-cell RNA sequence CRC data of normal epithelium,non-metastatic primary tumor,metastatic primary tumor,and liver metastases from gene expression omnibus(GEO)dataset were analyzed to reveal characteristics of CRC metastasis.Primary tu-mor tissues of three non-metastatic CRC and three metastatic CRC patients from Union Hospital of Tongji Medical College(Wuhan,China)were used to verify the characteristics of CRC metastasis.Results:We identified a metastasis-related cancer cell subset EP1,which was characterized with a high expression of KRT17,LAMC2,EMP1,and PLAC8.EP1 had an enhanced cell-cell interaction,which interacted with SPPþmacrophages and drove them toward anti-inflammatory and immunosuppressive phenotype.Dynamic changes in genes and TF regulons during the metastasis were also revealed.Conclusions:This study advanced our understanding of the development of tumor cells during CRC metastasis and further identi-fied metastasis-related subset and potential therapeutic targets for the treatment and prevention of CRC metastasis.

关 键 词：colorectal cancer METASTASIS single-cell RNA sequencing 

分 类 号：R735.34[医药卫生—肿瘤]