酚氨基改性MCM-41固定化脂肪酶的工艺优化及应用  

Process Optimization and Application of Lipase Immobilized on Phenol Amino Modified MCM-41

作　　者：李佥[1] 曾祥冰 孙西同 陈晓艺[1] 李苗 王添誉 黄帆 孙芳鸿 李宪臻[1] LI Qian;ZENG Xiangbing;SUN Xitong;CHEN Xiaoyi;LI Miao;WANG Tianyu;HUANG Fan;SUN Fanghong;LI Xianzhen(School of Biological Engineering,Dalian Polytechnic University,Dalian 116034,China;School of Light Industry and Chemical Engineering,Dalian Polytechnic University,Dalian 116034,China)

机构地区：[1]大连工业大学生物工程学院,辽宁大连116034 [2]大连工业大学轻工与化学工程学院,辽宁大连116034

出　　处：《食品工业科技》2025年第5期168-177,共10页Science and Technology of Food Industry

基　　金：国家自然科学基金青年基金项目(31601411,21804129,22108024);辽宁省教育厅基本科研项目(JYTMS20230423)。

摘　　要：为构建新型固定化脂肪酶催化体系并提高脂肪酶的稳定性,利用酚氨基改性有序介孔材料有序介孔分子筛(MCM-41)进行脂肪酶的固定化研究,并将固定化酶应用于实际反应体系。结果表明,固定化酶的最优工艺条件为:固定化温度30℃,固定化pH8.0,固定化时间7 h,初始酶浓度4 mg/mL,此时材料的载酶量为26.40 mg/g。固定化酶的最佳反应温度为50℃,最佳反应pH为7.0,酶活力最高可达4108±34.74 U/g载体。固定化酶重复使用8次后,保留了54.70%的初始酶活,在4℃条件下储藏30 d后仍具有71.10%的初始酶活。将固定化酶用于催化月桂酸己酯的合成,月桂酸转化率最高可达65.38%。酚氨基改性MCM-41固定化脂肪酶具有较高的酶活力和良好的稳定性,将其应用于实际催化反应中,具有较高的催化效率。进一步证明酚氨基改性是一种绿色、有效的改性策略,为酚氨基涂层的进一步应用提供了思路。In order to construct a new catalytic system of immobilized lipase and improve the stability of enzyme,the phenol amino modified ordered mesoporous material(MCM-41)was introduced for the study of lipase immobilization.The immobilized enzyme was also applied to the actual reaction system.The optimized conditions of lipase immobilization were as follows:Immobilization temperature of 30℃,immobilization pH of 8.0,immobilization time of 7 h,initial enzyme concentration of 4 mg/mL,and the enzyme loading was 26.40 mg/g.Meanwhile,the optimum reaction temperature of the immobilized enzyme was 50℃,and the optimum reaction pH was 7.0,together with the highest enzyme activity was 4108±34.74 U/g carrier.The immobilized enzyme was recycled for eight times,and it retained 54.70%of the initial enzyme activity.While after 30 days of storage at 4℃,it still kept 71.10%of the initial enzyme activity.In addition,the immobilized enzyme was used to catalyze the synthesis of hexyl laurate,and the highest conversion of lauric acid was 65.38%.It was indicated that the lipase immobilized on phenol amino modified MCM-41 exhibited high enzyme activity and good stability.When it was applied in the actual catalytic reaction,the immobilized enzyme displayed high catalytic efficiency.Furthermore,it was proved that phenol amino modification was a green and effective modification strategy,which could provide ideas for further application of phenol amino coating.

关 键 词：酚氨基改性 MCM-41 固定化脂肪酶 工艺优化 月桂酸己酯合成 

分 类 号：Q814.2[生物学—生物工程]