蛹虫草液体发酵菌株筛选及其胞外产虫草素、多糖条件的初步优化  

Screening of Excellent Strains in Liquid Fermentation for Cordyceps militaris and Preliminary Optimization of Extracellular Production Conditions for Cordycepin and Polysaccharides

作　　者：崔林虎 赵丽 孙闯 陈阳 姜立佳 汤萍 王莹 玄永男 CUI Linhu;ZHAO Li;SUN Chuang;CHEN Yang;JIANG Lijia;TANG Ping;WANG Ying;XUAN Yongnan(Yanbian Academy of Forestry,Yanji 133001,China)

机构地区：[1]延边林业科学研究院,吉林延吉133001

出　　处：《食药用菌》2025年第1期47-51,共5页Edible and Medicinal Mushrooms

基　　金：延边州科技攻关计划项目(2022NS20);延边州科技创新中心项目(2020ZH01)。

摘　　要：以液体发酵液中虫草素和多糖含量为指标对14个蛹虫草菌株进行筛选,发现菌株Y23液体发酵液中虫草素含量和多糖含量最高,进一步对其培养条件进行优化。结果表明:菌株Y23产虫草素和多糖,获得较高胞外产量的最佳培养条件分别为发酵液接种量10%~15%、静置培养温度22~28℃、静置培养时间20 d和接种量5%~15%、静置培养温度25~28℃、静置培养时间17 d。研究结果为蛹虫草生物活性成分工业化生产提供理论依据。Screening for the optimal strain among 14 Cordyceps militaris strains based on the content of cordycepin and polysaccharides in the liquid fermentation broth.The research found that strain Y23 exhibited the highest yields of cordycepin and polysaccharides in the liquid fermentation process.Further optimization of culture conditions was conducted,leading to the identification of optimal parameters:an inoculation rate of 10%-15%for maximum extracellular cordycepin content,a static culture temperature range of 22-28℃,and a static culture duration of 20 days.For higher-yield exopolysaccharides production,the optimal inoculation rate was determined to be 5%-15%,with a static culture temperature of 25-28℃ and a culture duration of 17 days.The results of this study provide a theoretical basis for the industrial production of bioactive components from C.militaris.

关 键 词：蛹虫草 液体发酵 优良菌株 虫草素 胞外多糖 

分 类 号：S646[农业科学—蔬菜学]