曲安奈德治疗口腔扁平苔藓的作用机制  

Mechanism of action of triamcinolone acetonide in the treatment of oral lichen planus

作　　者：赵爱敏 王春磊 李子涵 张良宇 张家玮 闫正明 刘雪 杜雨禾 Zhao Aimin;Wang Chunlei;Li Zihan;Zhang Liangyu;Zhang Jiawei;Yan Zhengming;Liu Xue;Du Yuhe(School of Stomatology Jining Medical College,Jining 272000,China)

机构地区：[1]济宁医学院口腔医学院,山东济宁272000

出　　处：《实用药物与临床》2025年第2期94-102,共9页Practical Pharmacy and Clinical Remedies

基　　金：教育部产学合作协同育人项目(230902368213435);济宁市重点研发计划项目(2021YXNS032);山东省大学生创新创业训练计划项目(S202210443019);济宁医学院大学生创新创业训练计划项目(cx2021026,cx2023025z)。

摘　　要：目的通过网络药理学和分子对接技术探究曲安奈德治疗口腔扁平苔藓(Oral lichen planus,OLP)的作用靶点及分子机制。方法通过PubChem、CTD、DrugBank、TargetNET、Pharm Mapper 5个药物数据库挖掘曲安奈德相关作用靶点,在GeneCards、Therapeutic Target Database、PharmGKB、DrugBank、OMIM和DisGeNET 6个疾病数据库中查找OLP的相关作用靶点,通过Venny在线工具绘制Venn图,获得曲安奈德与OLP共同的作用靶点;通过STRING构建“曲安奈德-OLP靶点”的蛋白互作(PPI)网络,应用Cytoscape 3.9.1软件预测曲安奈德治疗OLP的潜在关键靶点及核心靶点;通过DAVID数据库对潜在关键靶点进行GO以及KEGG富集分析;通过AutoDockTools和Autogrid4软件对核心靶点进行分子对接验证,并应用PyMol软件和LigPlot对分子结果进行可视化处理。结果筛选得到药物靶点386个,疾病靶点706个,5个曲安奈德治疗OLP的核心靶点(TNF、IL-1β、IL-6、CXCL8和IL-1α);通过GO以及KEGG富集分析得到有效的作用通路可能为人巨细胞病毒感染、糖尿病并发症中的AGE-RAGE信号通路等;分子对接结果表明,曲安奈德与核心靶点TNF、IL-1β、IL-6、CXCL8和IL-1α具有良好的结合活性。结论曲安奈德可通过多靶点和多通路共同治疗OLP,其机制可能与调节免疫炎症反应等有关。Objective To explore the targets and molecular mechanisms of triamcinolone acetonide in the treatment of oral lichen planus(OLP)through network pharmacology and molecular docking technology.Methods Five drug databases including PubChem,CTD,DrugBank,TargetNET and Pharm Mapper were used to explore the targets of action related to triamcinolone acetonide.The targets of action related to OLP in six disease databases including GeneCards,Therapeutic Target Database,PharmGKB,DrugBank,OMIM and DisGeNET were searched,and Venn diagrams were drawn by the Venny online tool to obtain the common targets of action between triamcinolone acetonide and OLP.Construct a protein-protein interaction(PPI)network of"triamcinolone acetonide-OLP targets"through STRING,and use Cytoscape 3.9.1 software to predict the potential key and core targets of triamcinolone acetonide in the treatment of OLP.Perform GO and KEGG enrichment analysis on potential key targets through the DAVID database.Molecular docking validation of core targets was performed using AutoDockTools and autogrid4 software,and the molecular results were visualized using PyMol software and LigPlot.Results Totally 386 drug targets,706 disease targets and 5 core targets for the treatment of OLP with triamcinolone acetonide(TNF,IL-1β,IL-6,CXCL8 and IL-1α)were identified through screening.The GO and KEGG enrichment analysis showed that the effective action pathway might be human cytomegalovirus infection,AGE-RAGE signaling pathway in diabetes complications and other pathways.The molecular docking results indicated that triamcinolone acetonide had good binding activity with core targets TNF,IL-1β,IL6,CXCL8,and IL-1α.Conclusion Triamcinolone acetonide can be used jointly in the treatment of OLP through multiple components,multiple targets,and multiple pathways,and its mechanism may be related to regulating immune inflammatory response.

关 键 词：曲安奈德 口腔扁平苔藓 网络药理学 分子对接 作用机制 

分 类 号：R781.5[医药卫生—口腔医学]