响应面法优化里氏木霉产β-葡聚糖酶的固态发酵工艺  

Optimization of Solid-State Fermentation Process for Producingβ-glucanase of Trichoderma reesei by Response Surface Methodology

作　　者：王娜 林青[1] 华兰兰 马贵军 娄恺[1] 霍向东[1] WANG Na;LIN Qing;HUA Lanlan;MA Guijun;LOU Kai;HUO Xiangdong(Institute of Microbiology,Xinjiang Academy of Agricultural Sciences,Xinjiang Laboratory of Special Environmental Microbiology,Xinjiang Urumqi 830091,China;Xinjiang Tiankang Feed Co.,Ltd.,Xinjiang Wujiaqu 831300,China)

机构地区：[1]新疆农业科学院微生物应用研究所,新疆特殊环境微生物实验室,新疆乌鲁木齐830091 [2]新疆天康饲料有限公司,新疆五家渠831300

出　　处：《饲料工业》2025年第3期104-112,共9页Feed Industry

基　　金：新疆生产建设兵团重点领域科技攻关计划项目[2022AB013]。

摘　　要：研究旨在优化里氏木霉ARTP-9(Trichoderma reesei ARTP-9)产β-葡聚糖酶工艺,为大规模固态发酵生产提供参考。采用单因素试验、Plackett-Burman试验、Box-Behnken响应面法优化里氏木霉ARTP-9固态发酵产β-葡聚糖酶工艺,分析接种方式、接种量、发酵基质、氮源、无机盐、表面活性剂、氮源含量、pH、水料比及发酵时间对产酶的影响。结果显示:固态种曲优化后孢子数为4.63×10^(8)CFU/g。最优产酶发酵工艺为基质中麸皮∶玉米芯粉=1∶1,硫酸铵2.5%,磷酸氢二钾1.67%,甜菜碱0.15%,液料比0.865∶1,固态种曲接种量18%,pH7.5,发酵4.6 d。其β-葡聚糖酶活性为66.32 U/g,较原始培养基提高1.34倍。响应面优化试验提高了里氏木霉ARTP-9产β-葡聚糖酶能力,酶制剂生产成本降低。证明该工艺具备良好的工业化生产应用前景。This research aimed to optimize theβ-glucanase production process of Trichoderma reesei ARTP-9 and provide a reference for large-scale solid-state fermentation production.The single factor experiment,Plackett-Burman experiment,and Box-Behnken response surface methodology were utilized to optimize the solid-state fermentation process of T.reesei ARTP-9 forβ-glucanase production.The effects of inoculation method and amount,fermentation substrate,nitrogen,inorganic salt,surfactant,nitrogen content,pH,water material ratio,and fermentation time on enzyme production were analyzed.The results show that:after optimization of the solid starter,the number of spores was 4.63×10^(8) CFU/g.The optimum fermentation process for enzyme production was as follows:solid substrate(wheat bran∶corn-core powder=1∶1),ammonium sulfate 2.55%,dipotassium phosphate 1.67%,betaine 0.15%,water material ratio 0.865∶1,solid seed inoculum 18%,pH 7.5,fermentation time 4.6 d.Under optimal fermentation conditions,β-gluca⁃nase activity reached 66.32 U/g,1.34 times higher than before optimization.Response surface optimization experiments improved the ability of T.reesei ARTP-9 to produceβ-glucanase and reduced enzyme production cost.These results demonstrate the process has good prospects for industrial production applications.

关 键 词：Β-葡聚糖酶 里氏木霉 固态发酵 响应面法 工艺优化 

分 类 号：S816.3[农业科学—饲料科学]