结核分枝杆菌Ⅱ型毒素-抗毒素系统VapBC家族的研究进展  

Advances in VapBC family of Mycobacterium tuberculosis typeⅡtoxin-antitoxin system

作　　者：付博 安能(综述) 郭法谋 孙红宾(审校) FU Bo;AN Neng;GUO Famou;SUN Hongbin(Department of Bioengineering and Biotechnology,School of Food and Bioengineering,Zhengzhou University of Light Industry,Zhengzhou 450000,Henan Province,China)

机构地区：[1]郑州轻工业大学食品与生物工程学院生物工程与生物技术系

出　　处：《中国生物制品学杂志》2025年第1期115-121,共7页Chinese Journal of Biologicals

基　　金：河南省自然科学基金(212300410415);河南省科技攻关项目(222102310603);郑州轻工业大学博士科研基金(2018-BSJJ021)。

摘　　要：结核分枝杆菌(Mycobacterium tuberculosis,Mtb)中有多种Ⅱ型毒素-抗毒素(toxin-antitoxin,TA)系统,其中VapBC家族是数量最多的TA系统,包括毒素VapC及其抗毒素VapB。毒素VapC具有PIN(PilT N-terminal)结构域特征,通常是核糖核酸酶(RNase),可通过切割tRNA或23S rRNA的次黄嘌呤-蓖麻毒素环(sarcin-ricin loop,SRL)发挥其毒性;抗毒素VapB可通过与启动子DNA结合调节同源毒素的表达水平或与同源毒素直接结合抑制其毒性。VapBC家族与Mtb的生长、致病性和耐药性等密切相关,因此,本文就Mtb中VapBC家族的功能、毒素VapC的底物特异性、VapBC的结构及基于该结构的药物研发现状作一综述,以期为治疗结核病(tuberculosis,TB)提供新的思路。There are many typeⅡtoxin-antitoxin(TA)systems in Mycobacterium tuberculosis(Mtb),among which the VapBC family is the most abundant TA system including toxin VapCs and antitoxin VapB.Toxin VapCs are usually ribonucleases(RNases)with a PilT N-terminal(PIN)domain that can cleave tRNA or sarcin-ricin loop(SRL)of 23S rRNA to exert the toxicity.Antitoxin VapB regulates the expression level of homologous toxins by binding to promoter DNA or directly binds to homologous toxins to inhibit their toxicity.The VapBC family is closely associated with the growth,pathogenicity and drug resistance of Mtb.Therefore,the function of Mtb VapBC family,substrate specificity of toxin VapCs,structures of VapBC,and structure-based drug development were summarized in this paper,in view to providing new ideas for the treatment of tuberculosis(TB).

关 键 词：结核分枝杆菌 毒素-抗毒素系统 VapBC家族 应激反应 蛋白结构 

分 类 号：R738.911[医药卫生—肿瘤]