马铃薯抗性淀粉对胰岛素抵抗的HepG2改善作用及机制  

Improvement effects and mechanism of potato resistant starch on insulin resistance in HepG2 cells

作　　者：李寒菊 樊树理[2] 邢宏宇 尹幸念[2] 吴建美 陈大方[1] LI Hanju;FAN Shuli;XING Hongyu;YIN Xingnian;WU Jianmei;CHEN Dafang(School of Public Health,Peking University,Beijing 100080;Toxicology Department,Inner Mongolia Comprehensive Disease Control and Prevention Center,Hohhot 010031)

机构地区：[1]北京大学公共卫生学院,北京100080 [2]内蒙古自治区疾病预防控制中心(内蒙古自治区预防医学科学院),呼和浩特010031

出　　处：《中国食品添加剂》2025年第1期59-66,共8页China Food Additives

基　　金：内蒙古自治区科技攻关计划资助项目(2021GG0120)。

摘　　要：采用液相色谱串联质谱法测定马铃薯抗性淀粉(resistant starch,RS)被消化后所得短链脂肪酸(short chain fatty acid,SCFA)构成比。高糖高胰岛素诱导建立胰岛素抵抗(insulin resistance,IR)HepG2细胞模型。通过检测不同浓度(0.01,0.1,1μmol/L)SCFA对IR HepG2细胞葡萄糖消耗量,及细胞内AMPK、Akt、PI3K、GLUT2、GLUT4蛋白表达和mRNA含量的影响,探究马铃薯RS消化所得SCFA对IRHepG2细胞改善作用及机制。结果表明:消化马铃薯RS产生的乙酸、丙酸、丁酸比例为2∶4∶1;葡萄糖30 mmol/L、胰岛素1μmol/L为IR造模适宜条件;SCFA能增加IR HepG2细胞葡萄糖消耗量,提高AMPK、AKT、PI3K、GLUT 2、GLUT 4蛋白和mRNA表达量。结论:马铃薯RS消化所得SCFA可以通过增强AMPK、PI3K/AKT信号通路和GLUT 2/GLUT 4靶蛋白对葡萄糖的转运,增加细胞葡萄糖消耗量,改善细胞IR,且起效浓度低、生物安全性高。High-performance liquid chromatography-tandem mass spectrometry was used to determine the composition of short-chain fatty acids(SCFAs)produced from the digestion of potato resistant starch(resistant starch,RS).An insulin-resistance(IR)model in HepG2 cell was established through high glucose and high insulin induction.To explore the improvement effects and mechanisms of SCFAs derived from potato RS on IR HepG2 cells,different concentrations of SCFA(0.01,0.1,1μmol/L)on glucose consumption,protein expression and mRNA content of AMPK,Akt,PI3K,GLUT 2 and GLUT 4 in IR HepG2 cells were assessed.The results showed that the ratio of acetic acid,propionic acid and butyric acid produced by potato RS digesting was 2∶4∶1.Glucose at 30 mmol/L and insulin at 1μmol/L were found to be suitable conditions for IR modeling.SCFAs increased glucose consumption and expression level of protein AMPK,AKT,PI3K,GLUT 2 and GLUT 4,as well as mRNA.In conclusion,SCFAs derived from the digestion of potato RS could improve IR in cells by enhancing glucose transport through the AMPK,PI3K/AKT signalling pathways and GLUT 2/GLUT 4 target proteins,increasing cellular glucose consumption,while exhibiting low effective concentrations and high biosafety.

关 键 词：马铃薯抗性淀粉 短链脂肪酸 2型糖尿病 胰岛素抵抗 HEPG2细胞 

分 类 号：TS202.1[轻工技术与工程—食品科学] TS202.3[轻工技术与工程—食品科学与工程]