Stem-leaf saponins of Panax notoginseng attenuate experimental Parkinson's disease progression in mice by inhibiting microglia-mediated neuroinflammation via P2Y2R/PI3K/AKT/NFκB signaling pathway  

作　　者：Hui Wu Chenyang Ni Yu Zhang Yingying Song Longchan Liu Fei Huang Hailian Shi Zhengtao Wang Xiaojun Wu 

机构地区：[1]Shanghai Key Laboratory of Compound Chinese Medicines,The Ministry of Education(MOE)Key Laboratory for Standardization of Chinese Medicines,Institute of Chinese Materia Medica,The MOE Innovation Centre for Basic Medicine Research on Qi-Blood TCM Theories,Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China

出　　处：《Chinese Journal of Natural Medicines》2025年第1期43-53,共11页中国天然药物(英文版)

基　　金：supported by the Educational Commission of Shanghai in China(No.2021LK114);the Organizational Key Research and Development Program of Shanghai University of Traditional Chinese Medicine(No.2023YZZ02);the Xinglin Young Talent Program at Shanghai University of Traditional Chinese Medicine(No.A1-U17205010430)。

摘　　要：Stem-leaf saponins from Panax notoginseng(SLSP)comprise numerous PPD-type saponins with diverse pharmacological properties;however,their role in Parkinson's disease(PD),characterized by microglia-mediated neuroinflammation,remains unclear.This study evaluated the effects of SLSP on suppressing microglia-driven neuroinflammation in experimental PD models,including the 1-methyl-4-phenylpyridinium(MPTP)-induced mouse model and lipopolysaccharide(LPS)-stimulated BV-2 microglia.Our findings revealed that SLSP mitigated behavioral impairments and excessive microglial activation in models of PD,including MPTP-treated mice.Additionally,SLSP inhibited the upregulation of inducible nitric oxide synthase(i NOS)and cyclooxygenase-2(COX2)and attenuated the phosphorylation of PI3K,protein kinase B(AKT),nuclear factor-κB(NFκB),and inhibitor of NFκB proteinα(IκBα)both in vivo and in vitro.Moreover,SLSP suppressed the production of inflammatory markers such as interleukin(IL)-1β,IL-6,and tumor necrosis factor alpha(TNF-α)in LPS-stimulated BV-2cells.Notably,the P2Y2R agonist partially reversed the inhibitory effects of SLSP in LPStreated BV-2 cells.These results suggest that SLSP inhibit microglia-mediated neuroinflammation in experimental PD models,likely through the P2Y2R/PI3K/AKT/NFκB signaling pathway.These novel findings indicate that SLSP may offer therapeutic potential for PD by attenuating microglia-mediated neuroinflammation.

关 键 词：Stem-leaf saponins of Panax Notoginseng P2Y2 receptor NEUROINFLAMMATION MICROGLIA NFΚB 

分 类 号：R285.5[医药卫生—中药学]