西藏不同地区枸杞果实表皮和叶际微生物多样性研究  

Microbial diversity of fruit epidermis and phyllosphere microbial diversity of Lycium chinense from different regions of Tibet

作　　者：奉综涛 买地那·帕尔哈提 尹秀 兰小中[1,2,3] 禄亚洲 FENG Zongtao;MAIDINA Paerhati;YIN Xiu;LAN Xiaozhong;LU Yazhou(College of Food Science,Xizang Agriculture and Animal Husbandry University,Linzhi 860000,China;The Provincial and Ministerial Co-founded Collaborative Innovation Center for R&D in Tibet Characteristic Agriculturaland Animal Husbandry Resources,Xizang Agriculture and Animal Husbandry University,Linzhi 860000,China;Key Laboratory of Tibetan Medicine Resources Conservation and Utilization of Tibet Autonomous Region,Xizang Agriculture and Animal Husbandry University,Linzhi 860000,China)

机构地区：[1]西藏农牧学院食品科学学院,西藏林芝860000 [2]西藏农牧学院西藏特色农牧资源研发省部共建协同创新中心,西藏林芝860000 [3]西藏农牧学院西藏自治区藏药资源保护与利用重点实验室,西藏林芝860000

出　　处：《中国酿造》2025年第2期150-157,共8页China Brewing

基　　金：西藏农牧学院研究生教育创新计划项目(YJS2023-06);第四次全国中药资源普查西藏自治区中(藏)药资源普查(20200501);西藏农牧学院学科建设项目(533323001);生物技术一流专业建设项目(2024-007)。

摘　　要：为探究西藏不同地区枸杞果实表皮及叶际微生物群落多样性,该研究采用高通量测序技术解析西藏林芝地区苦味红枸杞果实表皮(LH)、苦味红枸杞叶际(LHF)和日喀则地区红枸杞果实表皮(ZH)、红枸杞叶际(ZHF)微生物群落多样性,并对其优势细菌属和优势真菌属进行相关性分析。结果表明,枸杞叶际的细菌多样性和丰富度均高于果实表皮。在门水平上,变形菌门(Proteobacteria)为优势细菌门,子囊菌门(Ascomycota)为优势真菌门,其相对丰度分别为65.4%~90.6%和69.7%~95.6%。在属水平上,LH样品的优势细菌属是马赛菌属(Massilia)(35.1%);unclassified_f__Alcaligenaceae是LHF和ZHF样品的优势细菌属,其相对丰度分别为28.6%和49.6%;ZHF样品优势细菌属是泛菌属(Pantoea)(45.4%)。LH和LHF样品的优势真菌属是派伦霉属(Peyronellaea),其相对丰度分别为20.9%和73.9%;ZH样品的优势真菌属是短梗霉属(Aureobasidium)(30.8%);ZHF样品的优势真菌属是节丝壳属(Arthrocladiella)和小光壳属(Leptosphaerulina),其相对丰度分别为24.8%和23.4%,不同样品中优势微生物菌群及其相对丰度存在明显差异。相关性分析结果表明,优势细菌属和优势真菌属之间相互影响。In order to explore the fruit epidermis and phyllospheric microbial community diversity of Lycium chinense in different regions of Tibet,the microbial community diversity in bitter red L.chinense fruit epidermis(LH)and phyllosphere(LHF)in Nyingchi,Tibet,and red L.chinense fruit epidermis(ZH)and phyllospheric(ZHF)in Shigatse,Tibet was analyzed using high-throughput sequencing technology,and the correlation between their dominant bacterial genera and dominant fungal genera was analyzed.The results showed that the bacterial diversity and richness in the phyllosphere were higher than those in the fruit epidermis of L.chinense.At the phylum level,Proteobacteria was the dominant bacterial phylum,while Ascomycota was the dominant fungal phylum,with relative abundance ranging from 65.4%to 90.6%and 69.7%to 95.6%,respectively.At the genus level,the dominant bacterial genus in LH samples was Massilia(35.1%).Unclassified_f__Alcaligenaceae was the dominant bacterial genus in LHF and ZHF samples,with relative abundance of 28.6%and 49.6%,respectively.The dominant bacterial genus in the ZHF sample was Pantoea(45.4%).The dominant fungal genus in LH and LHF samples was Peyronellaea,with relative abundance of 20.9%and 73.9%,respectively.The dominant fungal genus in the ZH sample was Aureobasidium(30.8%),and the dominant fungal genera in ZHF samples were Arthrocladiella and Leptosphaerulina,with relative abundance of 24.8%and 23.4%,respectively.There were significant differences in the dominant microbial communities and their relative abundances among different samples.The correlation analysis results indicated that there were mutual influence between dominant bacterial genera and dominant fungal genera.

关 键 词：枸杞 表皮 叶际 微生物 群落多样性 相关性 

分 类 号：TS201.3[轻工技术与工程—食品科学]