发酵粘液乳杆菌HCS08-005冻干粉制备工艺优化及其抗过敏功能  

Optimization of preparation process and anti-allergic function of Limosilactobacillus fermentum HCS08-005 freeze-dried powder

作　　者：汤纯 徐长隆 宋佳 余萍 苏美玲 王小海 TANG Chun;XU Changlong;SONG Jia;YU Ping;SU Meiling;WANG Xiaohai(Jiangxi Renren Health Microecological Technology Co.,Ltd.,Yichun 331200,China)

机构地区：[1]江西仁仁健康微生态科技有限公司,江西宜春331200

出　　处：《中国酿造》2025年第2期158-163,共6页China Brewing

摘　　要：该研究以发酵粘液乳杆菌(Limosilactobacillus fermentum)HCS08-005为菌种,采用冷冻干燥技术制备冻干粉,通过单因素试验和响应面试验优化发酵粘液乳杆菌HCS08-005冻干粉的制备工艺,并通过斑马鱼试验验证该菌株对类胰蛋白酶表达水平的影响。结果表明,发酵粘液乳杆菌HCS08-005冻干粉的最佳制备工艺条件为:发酵温度37℃,发酵时间15 h,接种量5%,菌泥与冻干保护剂比例1∶3(kg∶L)。在此优化条件下,冻干粉活菌数为6.40×1011 CFU/g。斑马鱼对发酵粘液乳杆菌HCS08-005冻干粉的最大耐受质量浓度(MTC)为500μg/mL,且不同质量浓度的发酵粘液乳杆菌HCS08-005冻干粉均能极显著降低类胰蛋白酶表达水平(P<0.01)。因此,发酵粘液乳杆菌HCS08-005冻干粉具有抗过敏功效。In this study,using Limosilactobacillus fermentum HCS08-005 as the strain,the freeze-dried powder was prepared using freeze-drying technology.The preparation process of L.fermentum HCS08-005 freeze-dried powder was optimized through single factor and response surface experiments,and the effect of this strain on the expression level of trypsin was verified by zebrafish experiments.The results showed that the optimal process conditions for preparing L.fermentum HCS08-005 freeze-dried powder were as follow:fermentation temperature 37℃,fermentation time 15 h,inoculum 5%,and the ratio of bacterial mud and freeze-drying protective agent 1∶3(kg∶L).Under these optimized conditions,the viable bacterial count was 6.40×1011 CFU/g.Zebrafish tests results shown that the maximum tolerable concentration(MTC)of zebrafish to L.fermentum HCS08-005 freeze-dried powder was 500μg/ml,and different mass concentrations of L.fermentum HCS08-005 freeze-dried powder could significantly reduce the level of tryptase expression(P<0.01).The results showed that L.fermentum HCS08-005 freeze-dried powder had anti-allergic effect.

关 键 词：发酵粘液乳杆菌 冻干粉 制备工艺优化 响应面法 抗过敏 

分 类 号：TS202.1[轻工技术与工程—食品科学] Q939.97[轻工技术与工程—食品科学与工程]