BDNF基因rs6265多态性与青少年阈下抑郁患者认知功能障碍的相关性研究  

Correlation between BDNF gene rs6265 polymorphism and cognitive dysfunction in adolescents with subthreshold depression

作　　者：丁虎 周俊[1] 刘向来[1] 李佳[1] DING Hu;ZHOU Jun;LIU Xianglai;LI Jia(Psychological Counseling and Treatment Center,Hainan Anning Hospital,Haikou,Hainan 570100,China)

机构地区：[1]海南省安宁医院心理咨询治疗中心,海南海口570100

出　　处：《检验医学与临床》2025年第4期491-495,共5页Laboratory Medicine and Clinic

基　　金：海南省卫生计生行业科研项目(19A200061);海南省自然科学基金面上项目(819MS129)。

摘　　要：目的探讨脑源性神经营养因子(BDNF)基因rs6265多态性与青少年阈下抑郁患者认知功能障碍的相关性。方法选择2020年3月至2023年6月该院收治的216例青少年阈下抑郁患者作为研究对象,根据蒙特利尔认知评估量表(MoCA)评分结果分为障碍组141例(MoCA总评分<26分)、正常组75例(MoCA总评分≥26分)。取所有患者静脉血提取DNA,采用TaqMan探针单核苷酸多态性基因分型技术对BDNF基因rs6265的单核苷酸多态性进行检测,采用酶联免疫吸附试验检测血清BDNF水平。比较2组MoCA评分,rs6265各基因型、等位基因占比,以及血清BDNF水平。分析障碍组患者血清BDNF水平与MoCA总评分的相关性。结果障碍组患者MoCA总评分及各维度评分均低于正常组(P<0.05)。障碍组BDNF基因rs6265位点CC基因型占比明显低于正常组,TT基因型占比明显高于正常组,C等位基因占比明显低于正常组,差异均有统计学意义(P<0.05)。障碍组患者血清BDNF水平为(32.78±3.88)μg/L,明显低于正常组的(42.37±4.29)μg/L,差异有统计学意义(P<0.05)。障碍组患者血清BDNF水平与MoCA总评分呈正相关(r=0.755,P<0.001)。BDNF基因rs6265位点CC基因型患者血清BDNF水平明显高于TT基因型患者(t=6.282,P<0.001)。结论BDNF基因rs6265多态性与青少年阈下抑郁患者认知功能障碍有关,C等位基因和CC基因型占比下降可能与青少年阈下抑郁认知功能下降有关。Objective To investigate the correlation between brain-derived neurotrophic factor(BDNF)gene rs6265 polymorphism and cognitive dysfunction in adolescents with subthreshold depression.Methods A total of 216 adolescent patients with subthreshold depression admitted to this hospital from March 2020 to June 2023 were selected as study subjects.According to the Montreal Cognitive Assessment Scale(MoCA)scores,patients were divided into the cognitive impairment group(141 cases,the total MoCA score<26)and the normal group(75 cases,the total MoCA score≥26).DNA was extracted from the venous blood of all patients.The TaqMan probe single nucleotide polymorphism(SNP)genotyping technology was used to detect the BDNF gene rs6265 polymorphism.Serum BDNF level was detected by enzyme-linked immunosorbent assay.The MoCA scores between the two groups were compared along with the allele and genotype distribution of rs6265 and serum BDNF level.The correlation between serum BDNF level and the total MoCA score in the cognitive impairment group was analyzed.Results The total MoCA score and scores in each dimension of the patients in the cognitive impairment group were lower than those of the normal group(P<0.05).The proportion of patients with the CC genotype at the BDNF rs6265 locus was significantly lower,the proportion of genotype TT was significantly higher,and the proportion of allele C was significantly lower in the cognitive impairment group compared with the normal group,and the differences were all statistically significant(P<0.05).The serum BDNF level in the cognitive impairment group was(32.78±3.88)μg/L,which was significantly lower than that of(42.37±4.29)μg/L in the normal group,with a statistically significant difference(P<0.05).Serum BDNF level in the cognitive impairment group was positively correlated with the total MoCA score(r=0.755,P<0.001).The serum BDNF level in patients with the CC genotype at the BDNF rs6265 locus was significantly higher than that in patients with the TT genotype(t=6.282,P<0.001).Conclusion

关 键 词：脑源性神经营养因子基因 多态性 青少年 阈下抑郁 认知功能障碍 

分 类 号：R749.94[医药卫生—神经病学与精神病学] R446.9[医药卫生—临床医学]