Dendrobium officinale polysaccharide attenuates type 2 diabetes in mice model by modulating gut microbiota and alleviating intestinal mucosal barrier damage  

作　　者：Xiaoxia Chen Chun Chen Changyang Ma Wenyi Kang Junlin Wu Xiong Fu 

机构地区：[1]Guangdong Province Key Laboratory for Green Processing of Natural Products and Product Safety,Guangzhou 510640,China [2]SCUT-Zhuhai Institute of Modern Industrial Innovation,School of Food Science and Engineering,South China University of Technology,Guangzhou 510640,China [3]Guangzhou Institute of Modern Industrial Technology,Guangzhou 511458,China [4]Overseas Expertise Introduction Center for Discipline Innovation of Food Nutrition and Human Health(111 Center),Guangzhou 510640,China [5]National R&D Center for Edible Fungus Processing Technology,Henan University,Kaifeng 475004,China [6]Guangzhou Wondfo Health Science and Technology Co.,Ltd.,Guangzhou 510640,China

出　　处：《Food Science and Human Wellness》2025年第1期112-123,共12页食品科学与人类健康(英文)

基　　金：The Guangdong Basic and Applied Basic Research Foundation(2022A1515010730);the National Natural Science Foundation of China(32001647);the Science and Technology Plan Projects of Guangzhou City(202102100009)(the Guangzhou Key Laboratory for Intelligent Sensing and Quality Control of Agricultural Products);National Natural Science Foundation of China(31972022);the 111 Project(B17018)to conduct the project are gratefully acknowledged。

摘　　要：The purpose of this study was to investigate the hypoglycemic effect and mechanism of D endrobium officinale polysaccharide(DOP)on type 2 diabetes mellitus(T2DM)mice established by high-fat diet and streptozotocin.The results showed that DOP improved glycolipid metabolism and serum inflammation levels,and inhibited intestinal-derived lipopolysaccharide(LPS)translocation,suggesting that inhibiting LPS-mediated intestinal barrier damage may be a key target for DOP to alleviate T2DM.Interestingly,the study found that DOP reduced intestinal inflammation and oxidative stress levels,significantly up-regulated the mRNA expression of tight junction proteins Claudin-1,Occludin and zonula occluden-1(ZO-1),and ameliorated intestinal epithelial damage.In addition,DOP strongly inhibited the intestinal pathogenic bacteria and LPS-producing bacteria Helicobacter,Enterococcus and Desulfovibrio with a reduction rate of 95%,73% and 9%,respectively,and promoted the proliferation of anti-inflammatory bacteria Bifidobacterium and Lactobacillus by 139% and 8%,respectively.Taken together,the hypoglycemic effect of DOP was related to the protection of intestinal mucosal barrier,and its underlying mechanism lied in its excellent anti-inflammatory and gut microbiotamodulatory effects,providing a theoretical basis for developing DOP as a novel prebiotic in functional food for diabetes.

关 键 词：Dendrobium officinale polysaccharide HYPOGLYCEMIC MECHANISM LIPOPOLYSACCHARIDE Gut microbiota 

分 类 号：R151[医药卫生—营养与食品卫生学] R587.1[医药卫生—公共卫生与预防医学]