Molecular mechanism of exopolysaccharide biosynthesis regulated by wze and wzd genes on the immunomodulatory effects of probiotic Lacticaseibacillus paracasei S-NB  

作　　者：Luyao Xiao Qian Yang Changliang Zhang Kai Ma Xiaogan Zhao Xin Rui Wei Li 

机构地区：[1]Sanya Institute of Nanjing Agricultural University,College of Food Science and Technology,Nanjing Agricultural University,Nanjing 210095,China [2]Jiangsu New-Bio Biotechnology Co.,Ltd.,Jiangyin 214400,China [3]Jiangsu Biodep Biotechnology Co.,Ltd.,Jiangyin 214400,China

出　　处：《Food Science and Human Wellness》2025年第1期293-306,共14页食品科学与人类健康(英文)

基　　金：co-financed by the International Science&Technology Cooperation Program of Hainan Province(GHYF2023009);Jiangsu Provincial Key Research and Development Program(BE2022325);Postgraduate Research&Practice Innovation Program of Jiangsu Province(KYCX23_0777);Qing Lan Project of Jiangsu Province and Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD)。

摘　　要：The surface macromolecules of probiotic bacteria play crucial roles in modulating immune responses in the host.Exopolysaccharide(EPS)from lactic acid bacteria(LAB)have been widely reported to exhibit immunomodulatory activity.In this study,the EPS biosynthesis gene cluster of Lacticaseibacillus paracasei S-NB was analyzed and a deletion mutant S-NBΔ7576(two genes S-NB_2175/wze and S-NB_2176/wzd were responsible for the chain length determination and export of EPS)was successfully constructed,resulting a 40.02%decrease in the production of EPS.The deletion of wze and wzd had little effect on the monosaccharide composition and major groups of the two EPS fractions(BEPS1 and BEPS2).Both BEPS1 and BEPS2 could inhibit the transcriptional level of tumor necrosis factor-α(TNF-α),interleukin-1β(IL-1β),inducible nitric oxide synthase(i NOS)and cyclooxygenase-2(COX-2)mRNA in RAW 264.7 cells induced by lipopolysaccharide(LPS),and enhance host immune tolerance via suppressing NF-κB and MAPK signaling.Notably,the S-NBΔ7576 mutant supplied with the BEPS1/BEPS2 exhibited more significant inhibition of cytokines production and the phosphorylation of p65 and c-Jun N-terminal kinase(JNK)in LPS-stimulated cells compared with the S-NBΔ7576 mutant alone.Our study provided the immunomodulatory effect of BEPS1 and BEPS2 from L.paracasei S-NB,in which the wze and wzd genes associated with EPS biosynthesis may play an important role.

关 键 词：Lacticaseibacillus paracasei Exopolysaccharide(EPS) BIOSYNTHESIS IMMUNOMODULATORY 

分 类 号：TS201.3[轻工技术与工程—食品科学]