羧甲基化玉米芯菌多糖的结构表征及降血糖作用研究  

Structural Characterization and Hypoglycemic Effect of Carboxymethylated Corn Cob Polysaccharides

作　　者：陈小红[1] 许耿权 严建彬 温文英 林标声[1,4] CHEN Xiaohong;XU Gengquan;YAN Jianbin;WEN Wenying;LIN Biaosheng(College of Life Science,Longyan University,Longyan 364012,China;Yi Zhitai Biotechnology(Longyan)Co.,Ltd.,Longyan 364000,China;Quality Measurement Inspection and TestingCenter,Wuping County Market Supervisory Authority,Wuping 364300,China;Key Laboratory of Fujian Universities Preventive Veterinary Medicine and Biotechnology,Longyan University,Longyan)

机构地区：[1]龙岩学院生命科学学院,福建龙岩364012 [2]易之泰生物科技(龙岩)有限公司,福建龙岩364012 [3]武平县质量计量检验检测中心,武平县市场监督管理局,福建武平364300 [4]预防兽医学与生物技术福建省高等学校重点实验室,福建龙岩364012

出　　处：《三明学院学报》2024年第6期8-15,共8页Journal of Sanming University

基　　金：福建省大学生创新创业训练计划项目(202311312034);福建省省科技厅引导性项目(2023Y61010241)。

摘　　要：制备了羧甲基玉米芯多糖(carboxymethyl corncob polysaccharide,CCP),并对其进行了纯度鉴定、理化性质分析、结构鉴定及降血糖作用研究。结果表明:所制备的CCP水溶性增强,羧甲基取代度为0.0528,分子量160~180 kDa,比旋度[α]_(D)^(25)10°,黏度[η]32.23。CCP为葡萄糖、阿拉伯糖、半乳糖和木糖组成的多糖,其糖苷键连接方式可能为β(1→4)及β(1→6)连接,糖环为吡喃型。不同浓度的CCP均能降低试验大鼠的空腹血糖含量和血液中TC、TG、LDC-C等血脂指标的含量,且呈现一定的量效关系,其最适添加剂量为250 mg/(kg·d)。The carboxymethyl corncob polysaccharide(CCP)was prepared and its purity,physicochemical properties,structure,and hypoglycemic effect was studied in this paper.The results showed that the prepared CCP had enhanced water solubility,a degree of carboxymethyl substitution of 0.0528,a molecular weight of 160~80 kDa,and a specific rotation[α]_(D)^(25)1010°viscosity[η]32.23.CCP was a polysaccharide composed of glucose,arabinose,galactose and xylose,and its glycosidic bond connection mode may beβ(1→4)andβ(1→6)Connection,the sugar ring was pyran type.Different concentrations of CCP can reduce the fasting blood glucose content and blood lipid indicators such as TC,TG,and LDC-C in experimental rats,and exhibit a certain dose-effect relationship.The optimal additive dosage was 250 mg/(kg·d).

关 键 词：羧甲基 玉米芯 多糖 结构表征 降血糖 

分 类 号：TS210.9[轻工技术与工程—粮食、油脂及植物蛋白工程]