多指标正交试验探究炮制工艺对蜜炙桑叶品质的影响  

Effect of Processing Conditions on the Quality of Honey-roasted Mori Folium Evaluated by Multi-index Orthogonal Test

作　　者：王润丰 俞燕芳 王军文 黄金枝 闵艳艳 陈紫梅 陈宇欢 WANG Runfeng;YU Yanfang;WANG Junwen;HUANG Jinzhi;MIN Yanyan;CHEN Zimei;CHEN Yuhuan(School of Pharmacy,Jiangxi University of Chinese Medicine,Nanchang 330004,China;Jiangxi Cash Crops Research Institute,Nanchang 330046,China)

机构地区：[1]江西中医药大学药学院,江西南昌330004 [2]江西省经济作物研究所,江西南昌330046

出　　处：《现代中药研究与实践》2024年第6期54-60,共7页Research and Practice on Chinese Medicines

基　　金：国家现代农业产业技术体系建设专项(CARS-18);江西省自然科学基金(20212BAB215023)

摘　　要：目的探究炮制过程中蜜炙工艺对蜜炙桑叶品质的影响。方法采用单因素试验和多指标正交试验,考察烘制温度、烘制时间和加蜜量对蜜炙桑叶中总酚、总黄酮、体外抗氧化活性和美拉德反应副产物丙烯酰胺、5-羟甲基糠醛(5-HMF)生成等指标的影响。结果综合上述指标,发现烘制温度175℃、烘制时间55 min、加蜜量30%为最佳工艺,且该工艺下未检出丙烯酰胺和5-HMF。然而,蜜炙工艺造成生桑叶中原本的酚酸和黄酮类物质降解,并产生新的未知物质,这些物质也可能具有抗氧化活性。结论蜜炙工艺在考察条件下未生成有害美拉德反应产物,较为安全,新生成物质可作为蜜炙桑叶潜在的活性成分进一步研究。Objective To investigate the effect of processing conditions on the quality of honey-roasted mulberry leaves(Mori Folium).Methods Single factor test and multi-index orthogonal test were used to evaluate the effects of baking temperature,baking time and honey concentration on several indexes including total phenolics,total flavonoids,anti-oxidant activity and the by-products of Maillard reaction:acrylamide and 5-hydroxymethylfurfural(5-HMF)on honey-roasted mulberry leaves.Results Baking temperature at 175℃,baking for 55 min and honey concentration at 30%were the best parameters in this study.Both acrylamide and 5-HMF were not detected under this processing condition.However,the phenolic acids and flavonoids in raw mulberry leaves were sharply degraded after honey-roasted,and some new substances which may have antioxidant activities were produced in large quantities.Conclusion The processing conditions in this study is safe for producing honey-roasted mulberry leaves.The new compounds generated during baking can be further studied as potential bioactive components in honey-roasted mulberry leaves.

关 键 词：蜜炙桑叶 总酚 黄酮 抗氧化活性 美拉德反应 

分 类 号：R284.1[医药卫生—中药学]