具有潜在益生特性开菲尔慢生乳杆菌的筛选及全基因组分析  

Screening and whole-genome analysis of potential probiotic strain of Lentilactobacillus kefiri

作　　者：刘湘芸 赵鑫 王会莹 袁保壹 薄晓宇 包秋华[1] LIU Xiangyun;ZHAO Xin;WANG Huiying;YUAN Baoyi;BO Xiaoyu;BAO Qiuhua(Key Laboratory of Dairy Biotechnology and Engineering of Ministry of Education,Key Laboratory of Dairy Processing of Ministry of Agriculture and Rural Areas,Inner Mongolia Agricultural University,Key Laboratory of Dairy Biotechnology and Engineering of Inner Mongolia Autonomous Region,Hohhot 010018,China)

机构地区：[1]内蒙古农业大学乳品生物技术与工程教育部重点实验室,农业农村部奶制品加工重点实验室,内蒙古自治区乳品生物技术与工程重点实验室,呼和浩特010018

出　　处：《中国乳品工业》2025年第2期5-13,共9页China Dairy Industry

基　　金：内蒙古自治区自然科学基金项目(2023MS03046)。

摘　　要：为挖掘传统发酵乳制品中具有潜在益生特性的开菲尔慢生乳杆菌(Lentilactobacillus kefiri,L.kefiri)菌株。文章对10株L.kefiri进行体外益生特性检测,结果表明,L.kefiri NM119-2相较于其他菌株,益生性能良好,该菌株在pH=3的模拟人工胃液中3 h存活率为57.55%,在0.3%胆盐中4 h存活率为95.79%;对氯霉素、青霉素、环丙沙星和庆大霉素等6种抗生素显示敏感;疏水性达80.36%;静置24 h后自凝聚能力为43.30%;DPPH自由基清除率为97.91%,羟自由基清除率为52.2%,超氧阴离子清除率为27.81%,综合抗氧化能力强。碳水化合物利用能力结果显示,L.kefiri NM119-2可利用D-葡萄糖、D-乳糖和D-果糖等9种糖。全基因组测序结果显示,L.kefiri NM119-2基因组全长为2.48 Mb,编码2611个基因,涵盖了氨基酸运输和代谢、碳水化合物代谢和磷酸化作用等相关基因;L.kefiri NM119-2耐药基因均位于染色体上,安全风险较低。To exploit the potential probiotic properties of Lentilactobacillus(L.)kefiri in traditional fermented dairy products.The probiotic properties of 10 strains of L.kefiri were tested in vitro,the results indicated that L.kefiri NM119-2 possessed superior probiotic properties compared to the other strains,with a 3 h survival rate of 57.55%,in artificial gastric juice at pH=3 and 95.79%in bile salts at 0.3%for 4 h.L.kefiri NM119-2 exhibited sensitivity to six antibiotics,including chloramphenicol,penicillin,ciprofloxacin and gentamicin.And it had a hydrophobicity of 80.36%,the auto-aggregation capacity was 43.30%after 24 h of standing,and it possessed strong antioxidant capacity,among them,the scavenging rate of DPPH was 97.91%,the scavenging rate of hydroxyl radicals was 52.2%,and the scavenging rate of superoxide anion was 27.81%.The results of carbohydrate utilization showed that L.kefiri NM119-2 could utilize nine types of sugars,including D-glucose,D-lactose and D-Fructose.Whole genome sequencing of L.kefiri NM119-2 was demonstrated.The whole genome sequencing results showed the total length of L.kefiri NM119-2 was 2.48 Mb,encoding 2611 genes,including genes related to amino acid transport and metabolism,carbohydrae metabolism and phosphorylation.The resistance genes of L.kefiri NM119-2 were all located on chromosomes,which is a low safety risk.

关 键 词：开菲尔慢生乳杆菌 筛选 益生特性 全基因组分析 

分 类 号：TS201.3[轻工技术与工程—食品科学]