桑叶抗氧化活性谱效关系研究  

Study on the Spectral Effect Relationship of Antioxidant Activity of Mori Folium

作　　者：刘梦文 邓巧玲 沈静[1] 陈宣世 肖辉[1] 徐维盛 LIU Mengwen;DENG Qiaoling;SHEN Jing;CHEN Xuanshi;XIAO Hui;XU Weisheng(School of Public Health,Xinjiang Medical University,Urumqi 830011,China;National Institute for Nutrition and Health,Chinese Center for Disease Control and Prevention,Beijing 100050,China)

机构地区：[1]新疆医科大学公共卫生学院,乌鲁木齐830011 [2]中国疾病预防控制中心营养与健康所,北京100050

出　　处：《医药导报》2025年第3期387-391,共5页Herald of Medicine

基　　金：新疆维吾尔自治区创新环境(人才、基地)建设专项—科技创新基地建设计划(资源共享平台建设)(PT2018);中国营养学会全民营养科研基金资助项目(No.CNS-NNSRG 2019-96)。

摘　　要：目的基于谱效关系初步确定桑叶抗氧化活性的物质基础。方法采用高效液相色谱(HPLC)法建立桑叶指纹图谱,以1,1-二苯基-2-三硝基苯肼(DPPH)自由基清除能力等指标评价桑叶抗氧化活性,并采用多元统计分析探究谱效关系,结合分子对接技术预测作用靶点。结果建立了13批桑叶的HPLC指纹图谱,共标记共有峰13个,通过对照品比对法指认出9种化合物,相似度为0.932~0.998。不同批次桑叶间抗氧化活性存在差异,谱效关系分析发现绿原酸、隐绿原酸、芦丁、异绿原酸B等化学成分对抗氧化活性贡献较大,可与黄嘌呤氧化还原酶(XOD)、一氧化氮合酶-1(NO-1)和过氧化物酶体增殖物激活受体-α(PPAR-α)等多个抗氧化蛋白靶点存在结合机制,提示桑叶可能通过多成分-多靶点发挥抗氧化活性。结论通过整合桑叶指纹图谱与抗氧化活性数据,确定了桑叶中各成分的抗氧化活性贡献,为阐明桑叶中抗氧化活性物质基础及制定质量控制方法提供了实验依据。Objective To determine the material basis of the antioxidant activity of Mori Folium by examining the spectrum-effect relationship.Methods High-performance liquid chromatography(HPLC)was utilized to establish the fingerprints of Mori Folium.The antioxidant activity of Mori Folium was assessed using the 1,1-diphenyl-2-picrylhydrazyl(DPPH)radical scavenging assay and other related indicators.The spectrum-effect relationship of antioxidation was analyzed using gray relational analysis,bivariate correlation analysis,and partial least squares regression analysis.Molecular docking techniques were employed to predict potential interaction targets.Results HPLC fingerprints for 13 batches of Mori Folium were established,and thirteen common peaks were marked,with similarities ranging from 0.932 to 0.998.Nine common peaks were identified by comparing them to reference substances.Differences in antioxidant activity were observed among the different batches of Mori Folium.Based on the analysis of the spectrum-effect relationship,chemical components such as chlorogenic acid,cryptochlorogenic acid,rutin,and isochlorogenic acid B were found to contribute significantly to the antioxidant activity.These components may exert their effects by binding to several antioxidant protein targets,such as XOD,NO-1,and PPAR-α.This implies that Mori Folium might exert its antioxidant action via multiple components and targets.Conclusions By integrating the fingerprint and antioxidant activity of Mori Folium,the contributions of individual components to its antioxidant activity were determined.This study provides an experimental basis for elucidating the substances responsible for the antioxidant activity of Mori Folium and for establishing quality control methods.

关 键 词：桑叶 指纹图谱 谱效关系 抗氧化活性 分子对接 

分 类 号：R932[医药卫生—生药学] R284.1[医药卫生—药学]