长林小蠹气味降解酶基因的鉴定与分子对接  

Identification and molecular docking of odorant-degrading enzyme genes in Hylurgus ligniperda

作　　者：李相淳 陶静 李心钰 宗世祥[1] Li Xiangchun;Tao Jing;Li Xinyu;Zong Shixiang(School of Forestry,Beijing Forestry University,Beijing 100083,China)

机构地区：[1]北京林业大学林学院,北京100083

出　　处：《北京林业大学学报》2025年第2期79-94,共16页Journal of Beijing Forestry University

基　　金：国家重点研发计划(2021YFD1400300)。

摘　　要：【目的】对长林小蠹的气味降解酶相关基因进行筛选、分类与组织表达分析,为后续利用分子生物学手段调控长林小蠹的行为提供理论依据。【方法】在长林小蠹全基因组数据中筛选关键的气味降解酶基因,并进行基因的系统发育分析与成虫的组织表达分析。利用同源建模预测气味降解酶基因的三维结构,对模型结构进行合理性评估后,使用Autodock1.5.7软件进行分子对接的模拟。【结果】在长林小蠹全基因组数据中共鉴定出65个候选的气味降解酶基因,其中谷胱甘肽-S-转移酶的数量为11个,细胞色素P450的数量为46个,羧酸酯酶的数量为8个。系统发育结果显示,长林小蠹的气味降解酶基因相对保守。成虫的组织表达分析结果显示,长林小蠹气味降解酶相关基因主要在触角中表达,且谷胱甘肽-S-转移酶与细胞色素P450两类基因家族的表达量较高。分子对接结果显示,长林小蠹气味降解酶和不同气味分子之间的结合能普遍较低且差异较小,形成的分子间作用力较为固定。【结论】在长林小蠹的触角中显著表达的气味降解酶基因类型主要为谷胱甘肽-S-转移酶与细胞色素P450,分子对接的结果证明长林小蠹的气味降解酶基因具有普遍降解气味分子的能力,且分子间的结合方式相似。[Objective] This paper aims to screen,classify,and analyze the tissue expression of odor degradation enzyme-related genes in Hylurgus ligniperda.The results of this study will provide a theoretical foundation for utilizing molecular biology techniques to effectively regulate the behavior of Hylurgus ligniperda.[Method] Key odor-degrading enzyme genes were screened from the whole-genome data of Dendroctonus longipennis.Phylogenetic analysis of the genes and tissue expression analysis of the adults were conducted.Homology modeling was used to predict the three-dimensional structure of the odordegrading enzyme genes.After the rationality of the model structure was evaluated,molecular docking simulation was performed using Autodock1.5.7 software.[Result] The analysis revealed a total of 65potential odor degradation enzyme genes within the complete genome data of Hylurgus ligniperda.Among them,11 were identified as glutathione S-transferase genes,46 as cytochrome P450 genes,and 8 as esterase genes.The phylogenetic analysis displayed a relatively conserved nature of odor degradation enzyme genes in Hylurgus ligniperda.Furthermore,the gene expression distribution in adult beetle tissues indicated that odor degradation enzyme-related genes were predominantly expressed in the antennae,with higher levels of expression observed in the glutathione S-transferase and cytochrome P450 gene families.Notably,the molecular docking results demonstrated that the binding energies between the odor degradation enzymes of Hylurgus ligniperda and various odor molecules were consistently low and exhibited minimal differences,indicating a stable intermolecular interaction force.[Conclusion] The significant expression of glutathione S-transferase and cytochrome P450 genes in antennae of Hylurgus ligniperda reinforces their vital role in odor degradation.Furthermore,the molecular docking results confirm the universal ability of odor degradation enzyme genes of Hylurgus ligniperda in breaking down odor molecules,with similar intermolecular bin

关 键 词：长林小蠹 气味降解酶 基因鉴定 同源建模 分子对接 害虫防治 

分 类 号：S763.38[农业科学—森林保护学]