辽河口湿地不同植被类型根际土壤微生物群落结构及多样性分析  

Analysis of Rhizosphere Soil Microbial Community Structure and Diversity of Different Vegetation Types in Liaohe Estuary Wetland

作　　者：岳航宇 郭成久[1] 苏芳莉[1,3,4,5] 魏超 YUE Hangyu;GUO Chengjiu;SU Fangli;WEI Chao(College of Water Conservancy,Shenyang Agricultural University,Shenyang 110866,P.R.China;Jilin Institute of Soil and Water Conservation Research,Changchun 130033,P.R.China;Liaoning Panjin Wetland Ecosystem National Observation and Research Station,Panjin 124000,P.R.China;Liaoning National Positioning Observation and Research Station of Shuangtai Estuary Wetland Ecosystem,Panjin 124000,P.R.China;Liaoning Provincial Key Laboratory of Soil Erosion Control and Ecological Restoration,Shenyang 110866,P.R.China)

机构地区：[1]沈阳农业大学水利学院,辽宁沈阳110866 [2]吉林省水土保持科学研究院,吉林长春130033 [3]辽宁盘锦湿地生态系统国家野外科学观测研究站,辽宁盘锦124000 [4]辽宁双台河口湿地生态系统国家定位观测研究站,辽宁盘锦124000 [5]辽宁省水土流失与生态修复重点实验室,辽宁沈阳110866

出　　处：《生态环境学报》2025年第2期222-232,共11页Ecology and Environmental Sciences

基　　金：国家重点研发计划项目(2022YFF1301004);国家自然科学基金(青年)项目(32001370);辽宁省教育厅项目(JYTPT2024001)。

摘　　要：湿地是重要生态系统,微生物对维持其生态稳定发挥着重要作用。了解辽河口湿地不同植被类型根际土壤微生物群落结构和多样性特征对于揭示该区域植被与土壤的相互作用具有重要科学意义。以辽河口湿地为研究对象,选取芦苇分布区-芦苇根际土壤(D1)、芦苇-盐地碱蓬交错区-芦苇根际土壤(D2)、芦苇-盐地碱蓬交错区-盐地碱蓬根际土壤(D3)、盐地碱蓬分布区-盐地碱蓬根际土壤(D4)以及潮滩沉积物(D5),利用Illumina Miseq PE300高通量测序技术进行16S rRNA基因测序分析不同植被类型根际土壤微生物的多样性和群落结构特征,并探究土壤理化因子与其之间的关系。研究结果表明,1)辽河口湿地根际微生物检测出40门,95纲,184目,311科,528属,888种。2)在门水平上,变形菌门是最优势菌群。3)湿地各采样区域土壤微生物群落多样性分析表明,盐地碱蓬分布区-盐地碱蓬根际土壤微生物群落丰富度和多样性最高。4)冗余分析结果表明,土壤总氮、有机质、氯离子含量是影响土壤微生物群落结构的主要因素。研究成果可从微生态学角度为中国北方滨海湿地生态系统功能的研究和保护提供参考。Rhizosphere microbial communities play a crucial role in wetland ecosystems by interacting with plant roots and influencing various aspects of plant growth,including nutrient uptake,resistance to environmental stressors,and the overall ecosystem function.These communities are complex and dynamic,driven by interactions between microorganisms and plants,and further influenced by soil physicochemical properties and environmental conditions.The rhizosphere is a hotspot for microbial activity,where microbes help plants acquire nutrients,degrade organic matter,and provide resistance to pathogens.Understanding the diversity,structure,and functional roles of these microbial communities across different wetland types is essential to understand the ecological processes that support wetland biodiversity,ecosystem stability,and resilience.Wetlands are valuable ecosystems that provide essential services such as carbon sequestration,water filtration,and habitat for biodiversity.In coastal wetlands where plants such as Phragmites australis and Suaeda salsa are commonly found,rhizosphere microbial communities are integral to the health of these ecosystems.Investigating the diversity and structure of these microbial communities across various wetland types,along with identifying the key factors that influence them,is crucial for understanding how these ecosystems function and are conserved in the face of ongoing environmental changes.The Liaohe Estuary Wetland,located in northern China,was selected as the research area for this study.This wetland encompasses a range of vegetation zones with distinct ecological characteristics.Soil samples were collected from five different wetland types:the Phragmites australis distribution zone(P.australis rhizosphere soil,D1),the interlaced P.australis and S.salsa zone(P.australis rhizosphere soil,D2),the interlaced P.australis and S.salsa zone(S.salsa rhizosphere soil,D3),the S.salsa distribution zone(S.salsa rhizosphere soil,D4),and tidal flat sediments(D5).These zones were selected based on

关 键 词：辽河口湿地 植被类型 高通量测序 土壤微生物 微生物多样性 群落结构 

分 类 号：X154.36[环境科学与工程—环境科学] X172