Tetrahydrocurcumin-induced apoptosis of hepatocellular carcinoma cells involves the TP53 signaling pathway,as determined by network pharmacology  

作　　者：Zhuo-Cong Bao Ye Zhang Zhao-Dong Liu Hui-Jun Dai Fu Ren Ning Li Shang-Yu Lv Yan Zhang 

机构地区：[1]Graduate School,Shenyang Medical College,Shenyang 110034,Liaoning Province,China [2]Guangxi Medical University Cancer Hospital,Guangxi Medical University,Nanning 530021,Guangxi Zhuang Autonomous Region,China [3]Key Laboratory of Human Ethnic Specificity and Phenomics of Critical Illness in Liaoning Province,Shenyang Medical College,Shenyang 110034,Liaoning Province,China [4]Key Laboratory of Phenomics in Shenyang,Shenyang Medical College,Shenyang 110034,Liaoning Province,China [5]Department of Biochemistry,School of Basic Medicine,Shenyang Medical College,Shenyang 110034,Liaoning Province,China [6]Department of Clinical Medicine,Shenyang Medical College,Shenyang 110034,Liaoning Province,China [7]International Education School,International Exchange and Cooperation Office,Shenyang Medical College,Shenyang 110034,Liaoning Province,China

出　　处：《World Journal of Gastrointestinal Oncology》2025年第3期221-236,共16页世界胃肠肿瘤学杂志(英文)

基　　金：The Central Government Guides Local Science and Technology Development Fund,No.2023JH6/100100021;Liaoning Province Education Department Foundation of China,No.JYTMS20231393 and No.LJ212410164032;Scientific Research Project of the Liaoning Province Education Department,No.SYYX2019015;Science and Technology Foundation of Shenyang Medical College,No.20171004;the Science and Technology Innovation Fund for Master Students of Shenyang Medical College,No.Y20220509.

摘　　要：BACKGROUND Hepatocellular carcinoma(HCC)is a malignant disease with high incidence and mortality worldwide.This study focuses on the TP53 target protein to investigate the potential therapeutic effect of tetrahydrocurcumin(THC)on HCC and its mechanism of action.The research hypothesis is that THC can inhibit the proliferation,migration,and invasion of HCC cells,and promote their apoptosis by regulating the TP53 target protein.AIM To explore the mechanism by which THC inhibits HCC cell proliferation via the TP53 signaling pathway.METHODS Potential targets of THC and HCC were identified from multiple databases.The core targets were subjected to analyses using Gene Ontology and Kyoto Encyclopedia of Genes and Genomes databases,and visualization processing,using the online platform Metascape to identify the key molecules and signaling pathways involved in the action of THC against HCC.The molecular mechanisms of action of THC against TP53 in the inhibition of HCC cells were verified using cell counting kit-8,Transwell,apoptosis,and western blotting assays.RESULTS Molecular docking results showed that THC had a high score for the TP53 target protein.In vitro experiments indicated that THC effectively inhibited the proliferation and migration of HCC cells,and affected the expression levels of TP53,MDM2,cyclin B,Bax,Bcl-2,caspase-9,and caspase-3.CONCLUSION THC induces the apoptosis of HCC cells through the TP53 signaling pathway,thereby inhibiting their proliferation and migration.

关 键 词：Hepatocellular carcinoma cells TETRAHYDROCURCUMIN TP53 Network pharmacology Molecular docking 

分 类 号：R735.7[医药卫生—肿瘤]