To investigate the effect and mechanism of tetrahydrocurcumin on hepatocellular carcinoma based on phosphoinositide 3-kinases/AKT signaling pathway  

作　　者：Zhuo-Cong Bao Zhao-Dong Liu Ye Zhang Hui-Jun Dai Hui Jia Fu Ren Ning Li Lu Zhao Yi-Wei Wang Shang-Yu Lv Yan Zhang 

机构地区：[1]Graduate School,Shenyang Medical College,Shenyang 110034,Liaoning Province,China [2]Guangxi Medical University Cancer Hospital,Guangxi Medical University,Nanning 530021,Guangxi Zhuang Autonomous Region,China [3]School of Traditional Chinese Medicine,Shenyang Medical College,Shenyang 110034,Liaoning Province,China [4]Key Laboratory of Human Ethnic Specificity and Phenomics of Critical Illness in Liaoning Province,Shenyang Medical College,Shenyang 110034,Liaoning Province,China [5]Key Laboratory of Phenomics in Shenyang,Shenyang Medical College,Shenyang 110034,Liaoning Province,China [6]Department of Biochemistry,School of Basic Medicine,Shenyang Medical College,Shenyang 110034,Liaoning Province,China [7]Molecular Morphology Laboratory,College of Basic Medical Sciences,Shenyang Medical College,Shenyang 110034,Liaoning Province,China [8]Batch 2022,Clinical Medicine,Shenyang Medical College,Shenyang 110034,Liaoning Province,China [9]International Education School,International Exchange and Cooperation Office,Shenyang Medical College,Shenyang 110034,Liaoning Province,China

出　　处：《World Journal of Gastrointestinal Oncology》2025年第3期270-290,共21页世界胃肠肿瘤学杂志(英文)

基　　金：the Central Government Guidance Local Science and Technology Development Fund,No.2023JH6/100100021;Liaoning Province Education Department Foundation of China,No.JYTMS20231393;Scientific Research Project of Liaoning Province Education Department,No.SYYX2019015;Science and Technology Fund of Shenyang Medical College,No.20171004,Shenyang Medical College Master’s Science and Technology Innovation Fund,No.Y20220509;Liaoning Provincial Department of Education Scientific Research Project,No.LJ212410164032.

摘　　要：BACKGROUND Liver cancer has a high incidence and mortality worldwide,especially in China.Herein,we investigated the therapeutic effect and mechanism of tetrahydrocurcumin against hepatocellular carcinoma(HCC),with a focus on the of phosphoinositide 3-kinases(PI3K)/AKT signaling pathway.AIM To investigate the effects and mechanism of tetrahydrocurcumin in HCC cell lines HepG2 and Huh7.METHODS Using Metascape,we analyzed the potential targets of tetrahydrocurcumin in HCC.Molecular docking validation was performed using SYBYL2.0.Cell Counting Kit-8,wound healing,and transwell assays were performed to evaluate the effects of tetrahydrocurcumin on HepG2 and Huh7 cell migration,invasion,and apoptosis.The expression of PI3K/AKT signaling pathway-related proteins was detected by western blotting.RESULTS Network pharmacology and molecular docking showed that tetrahydrocurcumin has high binding affinity for phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha.In vitro experiments demonstrated that tetrahydrocurcumin suppressed the migration and invasion of liver cancer cells,promoted their apoptosis,and downregulated the expression of p-PI3K,p-AKT,and B cell leukemia/lymphoma 2,while upregulating caspase-3,p53,and B cell leukemia/lymphoma 2 associated X.CONCLUSION In summary,tetrahydrocurcumin suppresses PI3K/AKT signaling,promotes apoptosis,and prevents the migration and invasion of liver cancer cells.

关 键 词：Hepatocellular carcinoma TETRAHYDROCURCUMIN Network pharmacology Molecular docking PI3K/AKT signaling pathway 

分 类 号：R735.7[医药卫生—肿瘤]