利用构建的8505C-EGFP细胞研究蛹虫草对甲状腺癌化疗的辅助治疗作用  

Study on the Adjuvant Therapeutic Effects of Cordyceps militaris on Thyroid Cancer Chemotherapy Using the Constructed 8505C-EGFP Cell Line

作　　者：陈岚 高圆圆 王玉涛 张瑾 CHEN Lan;GAO Yuanyuan;WANG Yutao;ZHANG Jin(College of Life and Geographic Sciences,Key Laboratory of Biological Resources and Ecology of Pamirs Plateauin Xinjiang Uygur Autonomous Region,Kashi University,Kashi 844000,China;College of Biological and Chemical Engineering,Jiaxing University,Jiaxing 314001,China;Jiaxing i-Bio Biotechnology Co.,Ltd,Jiaxing 314050,China)

机构地区：[1]喀什大学生命与地理科学学院,新疆帕米尔高原生物资源与生态重点实验室,喀什844000 [2]嘉兴大学生物与化学工程学院,嘉兴314001 [3]嘉兴爱博生物科技有限公司,嘉兴314050

出　　处：《中国细胞生物学学报》2025年第2期177-184,共8页Chinese Journal of Cell Biology

基　　金：浙江省自然基金重点项目(批准号:LZ23C170002);喀什大学科研创新团队培育计划资助的课题。

摘　　要：该文构建了带有绿色荧光蛋白标记的甲状腺癌细胞系8505C-EGFP,以用于探究蛹虫草辅助化疗药物顺铂治疗甲状腺癌的作用。首先,将带有增强绿色荧光蛋白基因(EGFP)的质粒pEGFP-N2导入甲状腺癌细胞8505C,通过G418筛选结合细胞流式分选,获得稳定表达EGFP的甲状腺癌细胞系8505C-EGFP;然后,对比分析8505C和8505C-EGFP细胞的增殖能力、克隆形成能力和迁移能力,明确8505C-EGFP细胞特性;用8505C-EGFP评估顺铂(Cisplatin)和蛹虫草水提物(CM)联合用药对甲状腺癌细胞增殖、迁移和侵袭能力的影响;最后利用8505C-EGFP细胞注射构建裸鼠皮下移植瘤模型,在活体内验证顺铂和蛹虫草联合用药对甲状腺瘤的治疗效果。结果显示了8505C-EGFP细胞株的增殖、克隆形成和迁移能力与原始8505C细胞株相比无显著差异;顺铂和蛹虫草均能抑制8505C-EGFP细胞在体外的增殖、迁移及侵袭,但二者联合用药抑制作用显著优于二者单独使用;利用裸鼠皮下移植瘤模型证明了8505C-EGFP细胞在活体内具有示踪作用,并验证了在活体内顺铂和蛹虫草联合用药也能够显著抑制肿瘤的生长。综上所述,该研究成功构建了8505C-EGFP甲状腺癌细胞,利用该工具细胞证明了蛹虫草与顺铂联合用药对甲状腺瘤的显著抑制作用。This study established the 8505C-EGFP thyroid cancer cell line,labeled with EGFP(enhanced green fluorescent protein),to investigate the effects of Cordyceps militaris-assisted chemotherapy with Cisplatin in thyroid cancer treatment.The pEGFP-N2 plasmid carrying the EGFP gene was introduced into 8505C thyroid cancer cells.After selection with G418 and FACS(fluorescence-activated cell sorting),a stable 8505C-EGFP cell line with consistent EGFP expression was successfully generated.Next,the article conducted a comparative analysis of the proliferation,colony formation,and migration abilities of 8505C and 8505C-EGFP cells to characterize the properties of the 8505C-EGFP cell line.The 8505C-EGFP cells was used to evaluate the effects of combined treatment with Cisplatin and water extract of CM(Cordyceps militaris)on the proliferation,migration,and invasion of thyroid cancer cells.Finally,the subcutaneous xenograft model of nude mice was established by injecting 8505C-EGFP cells to validate the therapeutic efficacy of Cisplatin and Cordyceps militaris combination treatment on thyroid tumors in vivo.The results showed that the proliferation,colony formation,and migration abilities of the 8505C-EGFP cell line were not significantly different from those of the original 8505C cell line.Both Cisplatin and Cordyceps militaris inhibited the proliferation,migration,and invasion of 8505C-EGFP cells in vitro,but the combined treatment exhibited significantly stronger inhibitory effects compared with either treatment alone.Using the subcutaneous xenograft model in nude mice,8505C-EGFP cells was demonstrated to have a tracer effect in vivo and the combined treatment of Cisplatin and Cordyceps militaris also significantly suppressed tumor growth in vivo.In conclusion,this study successfully established the 8505C-EGFP thyroid cancer cell line and used this tool to demonstrate that the combination of Cordyceps militaris and Cisplatin has a pronounced inhibitory effect on thyroid tumors.

关 键 词：甲状腺癌 8505C 绿色荧光蛋白 蛹虫草 顺铂 

分 类 号：R736.1[医药卫生—肿瘤]