京尼平苷对盐碱胁迫下马铃薯试管苗渗透调节和抗氧化能力的影响  

Effects of Geniposide on Osmotic Regulation and Antioxidant Capacity of Potato Plantlets under Saline-alkali Stress

作　　者：潘阳 王立春[1] 田国奎[1] 王海艳[1] 李凤云[1] 庞泽 PAN Yang;WANG Lichun;TIAN Guokui;WANG Haiyan;LI Fengyun;PANG Ze(Keshan Branch of Heilongjiang Academy of Agricultural Sciences,Key Laboratory of Potato Biology and Genetics Breeding,Ministry of Agriculture and Rural Affairs,Heilongjiang Province Potato Germplasm Resources and Genetic Improvement Engineering Technology Research Center,Qiqihar 161600,Heilongjiang,China)

机构地区：[1]黑龙江省农业科学院克山分院,农业农村部马铃薯生物学与遗传育种重点实验室,黑龙江省马铃薯种质资源与遗传改良工程技术研究中心,黑龙江齐齐哈尔161600

出　　处：《中国蔬菜》2025年第2期148-153,共6页China Vegetables

基　　金：黑龙江省农业科技创新跨越工程农业科技基础创新优青项目(CX23YQ12);黑龙江省省属科研院所科研业务费资助项目(CZKYF2024-1-C003);黑龙江省农业科学院克山分院2023年度先导培育项目(XDZDA2023-01);黑龙江省农业科技创新跨越工程项目(CX23GG02);黑龙江省“揭榜挂帅”科技攻关项目(2022ZXJ06B02-02a,2022ZXJ06B01-03);国家马铃薯产业技术体系齐齐哈尔综合试验站项目(CARS-09-ES37);齐齐哈尔市科技计划联合引导项目(LNYGG-2024005)。

摘　　要：为明确京尼平苷在盐碱胁迫下对马铃薯生理生化机制的影响,以耐盐碱品种克新1号和盐碱敏感品种夏波蒂为研究对象,采用不同浓度的京尼平苷作用在盐碱胁迫下的马铃薯试管苗中,培育28 d,检测马铃薯试管苗叶片中渗透调节物质含量、细胞膜脂过氧化程度以及抗氧化防御系统中抗氧化酶活性与非酶抗氧化剂含量的变化。结果表明:在一定浓度范围内,京尼平苷处理能有效缓解盐碱胁迫对马铃薯试管苗的抑制程度;提高马铃薯试管苗叶片可溶性蛋白、可溶性糖以及脯氨酸含量,使渗透物质积累保持渗透调节平衡;降低丙二醛含量;提高抗氧化酶活性以及还原型谷胱甘肽含量。京尼平苷能提高马铃薯试管苗的抗盐碱胁迫能力,研究结果为京尼平苷应用于马铃薯抗逆生产提供理论基础。To study the effect of geniposide on the biochemical and physiological mechanism of potato under saline-alkali stress,the salt alkali tolerant variety‘Kexin No.1’and salt alkali sensitive variety‘Shepody’were studied in this study,the potaoto plantlets under saline-alkali stress were cultured with different concentrations of geniposide for 28 days.The contents of osmoregulatory substances and the degree of membrane lipid peroxidation of potato plantlets leaves were measured.Meanwhile,detecting changes in antioxidant enzymes activity and non-enzyme antioxidants content in the antioxidant defense system.The results showed that:in a certain range of concentration,geniposide treatment could effectively alleviate the inhibition degree of saline-alkali stress on potato plantlets in vitro.That can promote the increase of soluble protein,soluble sugar and proline content in the leaves of potato plantlets,allowing for the accumulation of osmotic substances and maintaining osmotic regulation balance.It reduces malondialdehyde content,and increase antioxidant enzyme activity and GSH content.The results indicated that geniposide could improve the saline-alkali stress resistance of potato plantlets in vitro.This study provides a theoretical basis for the application of geniposide in stress-resistant potato production.

关 键 词：盐碱胁迫 京尼平苷 马铃薯 渗透调节 抗氧化能力 

分 类 号：S532[农业科学—作物学]