食物多酚对未漂洗鱼糜中ATP降解的抑制作用研究  

Analysis of inhibitory effect of food polyphenols on ATP degradation in unrinsed surimi

作　　者：刘颖[1] 詹淼 陈亚泉 付湘晋[2] LIU Ying;ZHAN Miao;CHEN Yaquan;FU Xiangjin(Changsha Environmental Protection College,Changsha 410004;College of Food Science and Engineering,Central South University of Forestry and Technology,Changsha 410004;Hunan Xiangdian food Co.,Ltd.,Liuyang 410301)

机构地区：[1]长沙环境保护职业技术学院,长沙410004 [2]中南林业科技大学食品科学与工程学院,长沙410004 [3]湖南湘典食品有限公司,浏阳410301

出　　处：《中国食品添加剂》2025年第3期123-130,共8页China Food Additives

基　　金：国家自然科学基金面上项目(32372412);湖南省重点研发计划项目(2022NK2032);湖南省教育厅科研项目(22C1423)。

摘　　要：从14种食物多酚提取物中筛选出7种,对酸性磷酸酶(acid phosphatase,ACP)、黄嘌呤氧化酶(xanthine oxidase,XOD)抑制效果较好的食物多酚提取物,将其分别添加到未漂洗鱼糜中,测定未漂洗鱼糜的K值,并分析多酚单体对ACP、XOD的体外抑制活性。葡萄籽、石榴皮、蓝莓、橄榄、莲子皮的多酚提取物对ACP、XOD活性均有较高的抑制率。其中,石榴皮多酚提取物显著抑制草鱼未漂洗鱼糜K值的增长,当其添加量为2.0%时,冻融循环5次后,空白组和加样组的K值分别是(20.36±0.89)%和(10.41±0.50)%;石榴皮多酚提取物中安石榴苷对ACP和XOD的抑制效果呈剂量依赖性。说明石榴皮多酚提取物可有效抑制未漂洗鱼糜ATP降解。石榴皮多酚提取物是潜在的未漂洗鱼糜天然保鲜剂。Seven food polyphenol extracts exhibiting good inhibitory effects on acid phosphatase(ACP) and xanthine oxidase(XOD) were screened from 14 types of food polyphenol extracts.These extracts were subsequently added to unrinsed surimi to determine the K-value of the unrinsed surimi,and analyze the in vitro inhibitory activity of the polyphenol monomers on ACP and XOD.The results showed that the polyphenol extracts from grape seed,pomegranate peel,blueberry,olive,and lotus seed showed high inhibition rates against both ACP and XOD.Among these,pomegranate peel polyphenols extract significantly inhibited the increase in the K-value of unrinsed grass carp surimi.When added at a concentration of 2.0%,the K-values of the unrinsed grass carp surimi after five freeze-thaw cycles were(20.36±0.89)% for the control group and(10.41±0.50)% for the treated group.The typical polyphenol monomer,punicalagin,found in pomegranate peel polyphenol extract exhibited a dose-dependent inhibition effect on ACP and XOD.These findings indicated that pomegranate peel polyphenol extract effectively inhibited ATP degradation in unrinsed surimi,with punicalagin being a potentially active polyphenol monomer.Therefore,pomegranate peel polyphenol extract was considered a promising natural preservative for unrinsed surimi.

关 键 词：未漂洗鱼糜 酸性磷酸酶 黄嘌呤氧化酶 K值 安石榴苷 

分 类 号：TS254.4[轻工技术与工程—水产品加工及贮藏工程]