萝卜硫素对纳米二氧化钛诱导小鼠肝损伤的保护作用  

Protective Effect of Sulforaphane on Nano-Titanium Dioxide-Induced Liver Injury in Mice

作　　者：孔玉雯 朱金芳 李丽华 KONG Yuwen;ZHU Jinfang;LI Lihua(College of Food Science and Pharmacy,Xinjiang Agricultural University,Urumqi 830000;Department of Basic Teaching,School of Medicine,Taizhou University,Taizhou 318000,Zhejiang)

机构地区：[1]新疆农业大学食品科学与药学学院,乌鲁木齐830000 [2]台州学院医学院基础教研室,浙江台州318000

出　　处：《中国食品学报》2025年第1期160-168,共9页Journal of Chinese Institute Of Food Science and Technology

基　　金：浙江省自然科学基金项目(LY23H030002)。

摘　　要：目的:研究萝卜硫素(SFN)对纳米二氧化钛颗粒(TiO_(2) Nps)诱导的小鼠肝损伤的保护作用。方法:将50只6周龄雄性C57BL/6J小鼠随机分成对照组(NC)、TiO_(2) Nps模型组(100 mg/kg)、TiO_(2) Nps+低剂量SFN给药组(0.5 mg/kg)、TiO_(2) Nps+中剂量SFN给药组(1 mg/kg)、TiO_(2) Nps+高剂量SFN给药组(2 mg/kg),每组10只。9周后眼眶取血,留取小鼠肝脏。检测小鼠血清中ALT和AST水平,肝脏组织氧化应激、炎症指标。HE染色观察小鼠肝脏组织病理形态学变化,免疫组织化学染色检测小鼠肝脏中F4/80蛋白的表达。蛋白免疫印迹法检测OGG1、NF-κb、ERK、p-ERK蛋白表达水平。结果:与对照组相比,模型组小鼠血清中AST、ALT水平显著升高(P<0.01);与模型组相比,各SFN给药组均显著降低了小鼠血清中ALT、AST、TBIL水平(P<0.01);显著提高肝组织的GSH/GSSG比值以及SOD水平(P<0.01);显著降低小鼠肝脏组织MDA水平(P<0.05,P<0.01)。实时定量PCR结果显示,与模型组相比,SFN中、高剂量给药组显著降低了IL-6、TNF-α的mRNA水平(P<0.05,P<0.01)。Western Blotting结果显示,与模型组相比,给药组NF-κb、p-ERK、ERK蛋白表达水平显著下调(P<0.05,P<0.01)。HE染色结果显示,与模型组相比,给药组的肝细胞空泡化程度有所改善。免疫组织化学染色结果显示,相比于模型组,给药组F4/80表达减少。结论:SFN能够减轻TiO_(2) Nps诱导的小鼠肝损伤、氧化应激和炎症指标的变化,表明SFN能够缓解TiO_(2) Nps诱导的氧化应激和炎症反应,且可能通过NF-κb-ERK/p-ERK通路发挥作用。Objective:To investigate the protective effect of sulforaphane(SFN)against titanium dioxide nanoparticles(TiO_(2) Nps)-induced liver injury in mice.Methods:Fifty 6-week-old male C57BL/6J mice were randomly divided into a control group(NC),a TiO_(2) Nps model group(100 mg/kg),a TiO_(2) Nps+low-dose SFN administration group(0.5 mg/kg),a TiO_(2) Nps+medium-dose SFN administration group(1 mg/kg),a TiO_(2) Nps+high-dose SFN administration group(2 mg/kg),and 10 in each group.Blood was collected from the orbits after 9 weeks,and the livers of the mice were retained.The serum levels of ALT and AST,oxidative stress and inflammation were detected in the liver tissues of mice.HE staining was used to observe the histopathological and morphological changes in the liver tissues of mice,and immunohistochemistry staining was used to detect the expression of F4/80 proteins in the livers of mice.Protein immunoblotting was used to detect the expression levels of OGG1,NF-κb,ERK and p-ERK proteins.Results:Compared with the control group,the serum levels of AST and ALT were significantly increased in the model group(P<0.01);compared with the model group,each SFN administration group significantly reduced the serum levels of ALT,AST and TBIL(P<0.01);significantly increased the GSH/GSSG ratio of the liver tissues as well as the level of SOD(P<0.01);and significantly reduced the level of MDA in the liver tissues of the mice(P<0.05,P<0.01).Real-time quantitative PCR results showed that compared with the model group,the mRNA levels of IL-6 and TNF-αwere significantly reduced in the SFN medium-and high-dose administration group(P<0.05,P<0.01).Western blotting results showed that compared with the model group,the protein expression levels of NF-κb,p-ERK and ERK were significantly down-regulated in the administration group(P<0.05,P<0.01).HE staining results showed that compared with the model group,the administration group had HE staining results showed that the vacuolization degree of hepatocytes in the administered group was improved com

关 键 词：萝卜硫素 纳米二氧化钛 肝损伤 

分 类 号：R28[医药卫生—中药学]