辣椒炭疽菌侵染对采后辣椒活性氧代谢的影响  

Effect of Colletotrichum capsici Infection on Active Oxygen Metabolism of Postharvest Pepper

作　　者：宋茜 帅良 沙金兰 宋慕波 刘雅欣 刘云芬 SONG Qian;SHUAI Liang;SHA Jinlan;SONG Mubo;LIU Yaxin;LIU Yunfen(School of Food and Bioengineering,Guangxi Key Laboratory of Health Care Food Science and Technology,Hezhou University,Hezhou 542899,Guangxi;School of Chemistry and Food Science,Nanchang Normal University,Nanchang 330023)

机构地区：[1]贺州学院食品与生物工程学院、广西康养食品科学与技术重点实验室,广西贺州542899 [2]南昌师范学院化学与食品科学学院,南昌330023

出　　处：《中国食品学报》2025年第1期281-292,共12页Journal of Chinese Institute Of Food Science and Technology

基　　金：广西自然科学基金项目(2023GXNSFBA026112);大学生创新创业训练计划项目(S202311838084)。

摘　　要：辣椒炭疽菌是采后辣椒主要的致病真菌之一,严重破坏辣椒的品质并缩短其贮藏期。以青圆椒为试验材料,以划伤接种辣椒炭疽菌为处理组,划伤接无菌水为对照组,探究在(28±1)℃,(90±5)%相对湿度下,贮藏时间对辣椒活性氧(ROS)代谢的影响。结果表明,与对照组相比,接种辣椒炭疽菌显著提高了辣椒病情指数,9 d时病情指数高达3.97(P<0.05)。辣椒经炭疽菌侵染后,细胞膜透性、MDA含量、总酚和类黄酮的积累量分别为对照组的1.44,5,1.26,1.24倍。在贮藏早期发生“氧化爆发”——H_(2)O_(2)含量和O_(2)^(-)产生速率增加,同时炭疽菌侵染促进了辣椒PPO、POD、SOD、APX活性增加,至贮藏9 d时,PPO、SOD和APX活性分别为对照组的22,1.09,1.67倍。CAT和PAL活性呈先增后降的变化趋势,其中PAL于5 d达到峰值,为对照的1.506倍。相关性分析表明,O_(2)^(-)产生速率、PPO、POD、SOD和APX活性与病情指数呈极显著正相关(P<0.01)。结合主成分分析可知,病原菌侵染激活了辣椒的防御系统,打破了辣椒氧化还原动态平衡,导致贮藏后期ROS积累量增大,进而使辣椒组织受损严重,衰老、腐败加剧,其中相较于划伤处理,辣椒炭疽菌侵染使辣椒氧化损伤更为严重。本研究阐明辣椒炭疽菌与辣椒活性氧代谢之间的关系,为进一步研究辣椒炭疽病致病机理及其防控措施提供理论依据。Colletotrichum capsici is one of the main pathomycete to the postharvest of pepper and destroying the quality shortens its storage period.The effects of storage at(28±1)°C and(90±5)%relative humidity on the metabolism of reactive oxygen species(ROS)in pepper were investigated by using green round pepper as the experimental material,scratching inoculated with C.capsici as the treatment group and scratching inoculated with sterile water as the control.The results showed that compared with the control group,the disease index of pepper inoculated with Colletotrichum capsici was significantly increased,and the disease index was as high as 3.97(P<0.05)at 9 d.The cell membrane permeability,MDA content,total phenol and flavonoid accumulation of pepper after infection with C.capsici were 1.44,5,1.26,1.24 times of the control group,respectively.The'oxidative burst'--H_(2)O_(2)content and O_(2)^(-)production rate increased in the early stage of storage,and the infection of C.capsici promoted the activities of PPO,POD,SOD and APX in pepper,and by 9 d of storage,the activities of PPO,SOD and APX were 22,1.09 and 1.67 times higher than those of the control group,respectively.The activities of CAT and PAL increased first and then decreased,and PAL reached the peak on the 5th day,which was 1.506 times that of the control.Correlation analysis showed that O_(2)^(-)production rate,PPO,POD,SOD and APX activities were significantly positively correlated with disease index(P<0.01).Combined with principal component analysis,it can be seen that pathogen infection activates the defense system of pepper,breaks the dynamic balance of redox in pepper,leads to the increase of ROS accumulation in the later stage of storage,which in turn causes serious damage to peppers'tissues,aging and rotting.Compared with scratch treatment,the infection of pepper anthracnose makes the oxidative damage of pepper more serious.This study elucidates the relationship between C.capsici and pepper active oxygen metabolism,and provides a theoretical basis for f

关 键 词：辣椒 辣椒炭疽菌 活性氧代谢 

分 类 号：TS255.1[轻工技术与工程—农产品加工及贮藏工程]