一株海氏肠球菌的分离鉴定及益生效果评价  

Isolation and Identification of a Strain of Enterococcus hirae and Evaluation of Its Probiotic Effect

作　　者：马雪莲[1,2] 刘丹 杨希 马睿 高强[1] MA Xue-lian;LIU Dan;YANG Xi;MA Rui;GAO Qiang(State Key Laboratory of Plateau Ecology and Agriculture,Qinghai University,Xining 810016;College of Eco-environmental Engineering,Qinghai University,Xining 810016)

机构地区：[1]青海大学三江源生态与高原农业国家重点实验室,青海西宁810016 [2]青海大学生态环境工程学院,青海西宁810016

出　　处：《微生物学杂志》2025年第1期51-58,共8页Journal of Microbiology

基　　金：国家自然科学基金项目(32060029)。

摘　　要：青海湖裸鲤(Gymnocypris przewalskii)是青海湖特有的高原冷水鱼类,其体表无鳞,在淡水洄游阶段容易被多种病原菌及寄生虫感染。因此,挖掘潜在肠道益生菌对青海湖裸鲤的保护和繁育具有重要意义。从青海湖裸鲤肠含物中通过分离筛选得到一株具有抑菌能力的菌株YC4,经16S rRNA测序鉴定为海氏肠球菌(Enterococcus hirae)。该菌株可以利用多种碳源,在胆盐质量分数为0.1%的厌氧条件下快速生长,对大肠埃希菌(Escherichia coli)、金黄色葡萄球菌(Staphylococcus aureus)等6种常见致病菌表现抑菌能力并且在培养物上清中可以检测到乙酸、丙酸、异丁酸、丁酸和戊酸等5种短链脂肪酸。人工回归感染实验初步证实海氏肠球菌YC4无致病性,可以作为青海湖裸鲤及其他鱼类肠道预防病原菌感染的益生菌。Gymnocypris przewalskii is kind of cold-water fish from highland of Lake Qinghai,which is susceptible to a variety of pathogenic bacteria and parasites during the freshwater migration stage.Isolating and screening facultative anaerobic strains from the gut microbiota of G.przewalskii,and selecting the potential probiotics to be used to regulate gut microbiota composition has significance for improving the survival rate of G.przewalskii.One potential strain was screened from gut microbiota of G.przewalskii,YC4,which had the best inhibitory effect on six common pathogenic bacteria such as Escherichia coli and Staphylococcus aureus,was selected and identified by 16S rRNA sequencing as Enterococcus hirae.The strain could utilize multiple carbon sources,grow fast under anaerobic conditions at 0.1%(Mass Concentration)bile salt concentration,and five short-chain fatty acids,including acetic acid,propionic acid,isobutyric acid,butyric acid and valeric acid,could be detected in the culture supernatant.The artificial regression infection experiment confirmed that it is non-pathogenic,and can be used as a probiotic for the regulation of gut microbiota of G.przewalskii and other fish.

关 键 词：青海湖裸鲤 肠道微生物 乳酸菌 抑菌活性 短链脂肪酸 

分 类 号：Q93-331[生物学—微生物学]