玉米浆对产黑色素短梗霉合成聚苹果酸的影响研究  

Study on the Effect of Corn Syrup on the Synthesis of Polymalic Acid by Aureobasidium melanogenum

作　　者：张琳 赵廷彬 齐璟僮 贾璐冰 陈宏毅 乔长晟[3] ZHANG Lin;ZHAO Tingbin;QI Jingtong;JIA Lubing;CHEN Hongyi;QIAO Changsheng(Tianjin Huizhi Biotrans Bioengineering Co.,Ltd.,Tianjin 300457,China;Tianjin Dairy Food Monitoring Center Co.,Ltd.,Tianjin 300381,China;College of Bioengineering,Tianjin University of Science&Technology,Tianjin 300457,China;Sichuan Food and Fermentation Industry Research&Design Institute Co.,Ltd.,Chengdu 611130,China)

机构地区：[1]天津慧智百川生物工程有限公司,天津300457 [2]天津市乳品食品监测中心有限公司,天津300381 [3]天津科技大学生物工程学院,天津300457 [4]四川省食品发酵工业研究设计院有限公司,成都611130

出　　处：《食品与发酵科技》2025年第1期56-64,共9页Food and Fermentation Science & Technology

基　　金：国家自然科学基金(No.22278412)。

摘　　要：聚苹果酸(PMLA)是一种新型的高分子聚合物材料,被广泛应用于医疗、环保、材料等领域。该研究发现,向基础发酵培养基中添加玉米浆有助于促进产黑色素短梗霉合成PMLA。结果表明,玉米浆添加浓度为7 g/L时,PMLA在5 L发酵罐中的含量可达到(69.73±1.20)g/L,相较于空白组提高了43.5%。通过转录组学分析,发现添加7 g/L玉米浆后,菌体糖酵解途径、尿素循环与TCA循环途径等相关基因表达均有所上调,从而提高发酵液中PMLA含量。Polymalic acid(PMLA)is a new type of polymer material,which has been widely applied in medical,environmental protection,materials and other fields.In this study,it was found that the addition of corn syrup in the basic fermentation medium helped to promote the synthesis of PMLA by Aureobasidium melanogenum.Results showed when the added concentration of corn syrup was 7 g/L,the content of PMLA in a 5 L fermentation tank could reach(69.73±1.20)g/L,which was 43.5%higher than that of the blank group.After transcriptomic analysis,it was found that after adding 7 g/L of corn syrup,the expression of related genes had been promoted such as bacterial glycolysis pathway,urea cycle,and TCA cycle pathway,thereby increased the content of PMLA.

关 键 词：聚苹果酸 玉米浆 产黑色素短梗霉 转录组分析 

分 类 号：TQ921.7[轻工技术与工程—发酵工程]