根霉酯化酶淀粉酶活力测定条件的优化  

Optimization of Conditions for Determination of Esterification Enzyme and Amylase Activity of Rhizopus

作　　者：陈定一 李俊琳 王丽玲[1] 侯旭杰[1] CHEN Dingyi;LI Junlin;WANG Liling;HOU Xujie(College of Food Science and Engineering,Tarim University,Key Laboratory of Southern Xinjiang Special Agricultural Products Deep Processing Corps,Aral,Xinjiang 843300,China)

机构地区：[1]塔里木大学食品科学与工程学院,南疆特色农产品深加工兵团重点实验室,新疆阿拉尔843300

出　　处：《农产品加工》2025年第4期45-50,共6页Farm Products Processing

基　　金：国家自然科学地区基金项目(32060526);兵团“强南”科技创新骨干人才项目(2021CB023);第三师图木舒克市科技计划项目(KY2021GG18)。

摘　　要：为了准确测定根霉产淀粉酶、酯化酶的能力,以高产酯化酶和淀粉酶的2株根霉为材料,对3,5-二硝基水杨酸法测定根霉淀粉酶、对硝基苯酚比色法测根霉酯化酶的方法进行了优化,建立了3,5-二硝基水杨酸(DNS)法测定根霉淀粉酶活力的方法和硝基苯酚法测酯化酶活力方法。该方法可为根霉淀粉酶、酯化酶活力测定提供一定的参考依据,也可为科学了解根霉代谢产物和食用菌工业发酵菌种的选育提供参考依据和分析方法。In order to accurately determine the ability of Rhizopus to produce amylase and esterase,two high-producing strains of Rhizopus were used as 3,5-dinitrosalicylic acid method for the determination of Rhizopus amylase and P-Nitrophenol colorimetric method for the determination of Rhizopus esterase were 3,5-dinitrosalicylic acid(DNS)method and nitrophenol method were used to determine the activity of Rhizopus amylase.This method could provide a certain reference basis for determining the activity of amylase and esterification enzyme of Rhizopus,and could also provide a reference basis and analysis method for scientifically understanding the metabolites of Rhizopus and the selection and breeding of industrial fermentation strains of edible Fungi.

关 键 词：酯化酶 淀粉酶 测定方法 根霉 方法优化 

分 类 号：TS261.11[轻工技术与工程—发酵工程]