贝莱斯芽孢杆菌G127的高密度发酵工艺优化  

Optimization of high-density fermentation process for Bacillus velezensis G127

作　　者：张根林[1] 胡可心 周超 杜晓盼 许世武 高波 ZHANG Genlin;HU Kexin;ZHOU Chao;DU Xiaopan;XU Shiwu;GAO Bo(College of Chemistry and Chemical Engineering/Key Laboratory of Green Process Corps of Chemical Engineering,Shihezi University,Shihezi 832003,Xinjiang,China;Woda Agricultural Technology Co.Ltd./Key Laboratory of Agricultural Microbiology and Pharmaceutical Fertilizer Creation Corps,Shihezi 832003,Xinjiang,China)

机构地区：[1]石河子大学化学化工学院/化工绿色过程兵团重点实验室,新疆石河子832003 [2]沃达农业科技股份有限公司/农用微生物与药肥创制兵团重点实验室,新疆石河子832003

出　　处：《西安工程大学学报》2025年第1期17-25,共9页Journal of Xi’an Polytechnic University

基　　金：兵团重点领域科技攻关计划(2022AB008,2022CB010-01)。

摘　　要：贝莱斯芽孢杆菌(Bacillus velezensis)分布广泛、生物活性高,是一种安全可靠的生物防治菌株,降低成本提高细胞生物量是实现Bacillus velezensis广泛应用的关键。采用单因素实验筛选最佳碳源、氮源和无机盐,通过响应面优化Bacillus velezensis G127的高密度发酵条件和工艺。结果表明:最佳培养基组成配方及培养条件为牛肉膏添加量15.2 g/L、糖蜜13.0 g/L、NaCl 5.0 g/L、发酵时间72 h、发酵温度30℃、接种量3.3%、初始pH值5.1,优化后生物量可达5.45×10^(10) CFU/mL,获得的高密度发酵工艺为Bacillus velezensis G127的规模化生产及应用提供保障。Bacillus velezensis is widely distributed and has high biological activity,which is a safe and reliable biocontrol strain.Reducing cost and increasing cell biomass is the key to its wide application.The optimal carbon source,nitrogen source and inorganic salt were selected by single factor experiment,and then the high-density fermentation conditions and process of Bacillus velezensis G127 were optimized by response surface.The optimal medium composition and culture conditions were as follows:beef paste supplemental level 15.2 g/L,molasses 13.0 g/L,sodium chloride 5.0 g/L,fermentation time 72 h,fermentation temperature 30℃,inoculation amount 3.3%,and initial pH value 5.1.The optimized biomass could reach 5.45×10^(10) CFU/mL.The obtained high-density fermentation technology provides guarantee for the large-scale production and application of Bacillus velezensis G127.

关 键 词：生物防治 贝莱斯芽孢杆菌(Bacillus velezensis) 响应面优化 高密度发酵 

分 类 号：S[农业科学]