荔枝果肉B型原花青素三聚体调节肝细胞脂代谢作用研究  

Effects of B-type proanthocyanidins trimer from Litchi chinensis Sonn pulp on regulating lipid metabolism in hepatocytes

作　　者：罗广怡 温叶杰 张瑞芬[2] 邓梅 张名位[2] 贾栩超 胡坤 LUO Guang-Yi;WEN Ye-Jie;ZHANG Rui-Fen;DENG Mei;ZHANG Ming-Wei;JIA Xu-Chao;HU Kun(College of Food Science,Guangdong Pharmaceutical University,Zhongshan 528400,China;Sericultural&Agri-food Research Institute Guangdong Academy of Agricultural Sciences,Key Laboratory of Functional Foods,Ministry of Agriculture,Guangdong Key Laboratory of Agricultural Products Processing,Guangzhou 510000,China)

机构地区：[1]广东药科大学食品科学学院,中山528400 [2]广东省农业科学院蚕业与农产品加工研究所,农业农村部功能食品重点实验室,广东省农产品加工重点实验室,广州510000

出　　处：《食品安全质量检测学报》2025年第5期69-75,共7页Journal of Food Safety and Quality

基　　金：2024年广东省级乡村振兴战略专项(2024KJ15);广东省特支计划本土创新团队项目(2019BT02N112)。

摘　　要：目的 探究荔枝果肉多酚主要活性组分B型原花青素三聚体抑制肝细胞甘油三酯(triglyceride, TG)沉积的剂量效应及其分子机制。方法 采用不同质量浓度(0.5~10.0μg/mL)B型原花青素三聚体处理油酸(oleic acid,OA)诱导的HepG2细胞脂肪变性模型,检测OA负荷肝细胞内TG含量及其与脂质吸收、转运、氧化和肝细胞凋亡相关基因表达。结果 0.5~10.0μg/mL B型原花青素三聚体均能显著抑制OA负荷肝细胞内TG沉积,但不呈现剂量依赖性;低剂量(1.0μg/mL) B型原花青素三聚体主要通过增强肝细胞Bcl-2和Bax表达量的比值,抑制肝细胞凋亡进而减轻细胞TG沉积;除上述途径外,中、高剂量(5.0μg/mL、10.0μg/mL) B型原花青素三聚体还可通过下调肝细胞CD36和FATP2的表达抑制细胞脂质吸收,同时上调肝细胞ACSL1和CPT1α的表达促进细胞脂质分解,进而减轻肝细胞TG沉积。结论 荔枝果肉B型原花青素三聚体可以通过抑制肝细胞脂质吸收,促进脂肪酸的β氧化以及抑制肝细胞过度凋亡,进而改善肝脏脂质代谢紊乱。Objective To explore the dose effects and molecular mechanism of B-type proanthocyanidins trimer,the main active component of Litchi chinensis Sonn pulp phenolics,on hepatocyte triglyceride(TG)deposition.Methods HepG2 cell steatosis model induced by oleic acid(OA)was treated with different mass concentrations(0.5–10.0μg/mL)of B-type proanthocyanidins trimer.The content of TG and the expressions of genes related to lipid absorption,transport and oxidation,together with apoptosis of hepatocytes,were detected in oleic acid loaded hepatocytes.Results B-type proanthocyanidins trimer(0.5–10.0μg/mL)all significantly inhibited TG accumulation in oleic acid-loaded hepatocytes,while no dose dependence was observed.Low-dose(1.0μg/mL)B-type proanthocyanidins trimer inhibited hepatocyte apoptosis by increasing the relative expression ratio of Bcl-2 to Bax,thereby reducing TG accumulation in hepatocytes.In addition to the above-mentioned pathway,medium/high-dose(5.0μg/mL,10.0μg/mL)B-type proanthocyanidins trimer also inhibited lipid absorption in hepatocytes by down-regulating CD36 and FATP2 expression,and promoted hepatocytes lipolysis by up-regulating ACSL1 and CPT1αexpression,hence reducing TG accumulation in hepatocytes.Conclusion B-type proanthocyanidins trimer of Litchi chinensis Sonn pulp can inhibit lipid absorption,promoteβ-oxidation of fatty acids and inhibit excessive apoptosis of liver tissue cells,thereby improving lipid metabolism and preventing fatty liver.

关 键 词：荔枝 B型原花青素三聚体 肝细胞脂肪变性 分子机制 

分 类 号：TS201.4[轻工技术与工程—食品科学]