芜菁多糖口服液的制备工艺研究  

Research on the preparation process of Brassica rapa L.polysaccharide oral liquid

作　　者：海力茜·陶尔大洪[1] 陶欣 王鑫 热依拉木·海力力 玉买热·阿布里孜 雅森·米吉提[3] HAILIXI Tao-Er-Da-Hong;TAO Xin;WANG Xin;REYILAMU Hai-Li-Li;YUMAIRE A-Bu-Li-Zi;YASHEN Mi-Ji-Ti(College of Pharmacy,Xinjiang Medical University,Urumqi 830017,China;Xinjiang Alinur Agricultural Science and Technology Development Co.,Ltd.,Urumqi 830017,China;College of International Education,Xinjiang Medical University,Urumqi 830017,China)

机构地区：[1]新疆医科大学药学院,乌鲁木齐830017 [2]新疆艾力努尔农业科技开发有限公司,乌鲁木齐830017 [3]新疆医科大学国际教育学院,乌鲁木齐830017

出　　处：《食品安全质量检测学报》2025年第5期112-118,共7页Journal of Food Safety and Quality

基　　金：新疆维吾尔自治区重大科技专项(2022A03007-3);新疆天然药物活性组分与释药技术重点实验室项目(XJDX1713);国家自然科学基金项目(81960765)。

摘　　要：目的 优化芜菁多糖口服液的制备工艺,并对其进行质量检测。方法 采用分级醇沉研究不同乙醇浓度下芜菁粗多糖的得率。通过单因素实验和正交实验筛选出芜菁多糖口服液的最佳配方,并根据药典规定进行质量检测。结果 乙醇浓度为20%、40%、60%和80%的醇沉组的芜菁粗多糖得率分别为12.8%、9.2%、23.2%和24.8%。芜菁精制多糖口服液最佳制备工艺条件为:芜菁精制多糖添加量2%,蜂蜜添加量15%,柠檬酸添加量0.6%,柠檬酸钠添加量0.6%。芜菁粗多糖口服液最佳制备工艺条件为:芜菁粗多糖添加量2%,蜂蜜添加量15%,柠檬酸添加量0.6%,柠檬酸钠添加量0.6%。芜菁精制多糖口服液pH和芜菁粗多糖口服液pH均在4~5。芜菁精制多糖口服液其相对密度为1.106 g/mL,芜菁粗多糖口服液其相对密度为1.100 g/mL。结论 芜菁精制多糖和芜菁粗多糖口服液的最佳制备工艺条件相同,前者的口味、气味以及外观均优于芜菁粗多糖口服液,但是芜菁精制多糖的提取工艺略显复杂,不适用于工厂批量生产。Objective To optimize the preparation process of Brassica rapa L.polysaccharide oral liquid and test its quality.Methods Graded alcohol precipitation was used to study the yield of crude polysaccharides of Brassica rapa L.at different ethanol concentrations.The optimal formulation of oral solution of Brassica rapa L.polysaccharide was screened by one-way and orthogonal tests,and the quality was tested according to the pharmacopoeia.Results The extraction rates of crude polysaccharide from Brassica rapa L.were 12.8%,9.2%,23.2%and 24.8%for the alcohol precipitation group with ethanol concentration of 20%,40%,60%and 80%,respectively.The optimal preparation process conditions of oral solution of Brassica rapa L.refined polysaccharide were:2%addition of Brassica rapa L.crude polysaccharide,15%addition of honey,0.6%addition of citric acid and 0.6%addition of sodium citrate.The best preparation process conditions for oral liquid of Brassica rapa L.crude polysaccharide were:2%addition of Brassica rapa L.crude polysaccharide,15%addition of honey,0.6%addition of citric acid,0.6%addition of sodium citrate.The pH of the oral solution of refined polysaccharide of Brassica rapa L.and the oral solution of crude polysaccharide of Brassica rapa L.were 4–5.The relative density of the oral solution of refined polysaccharide of Brassica rapa L.was 1.106 g/mL.The relative density of the oral solution of crude polysaccharide of Brassica rapa L.was 1.100 g/mL.Conclusion The optimal preparation process conditions for refined polysaccharides from Brassica rapa L.and crude polysaccharides from Brassica rapa L.are the same,but the taste,odor,and appearance of the former are superior to those of crude polysaccharides from Brassica rapa L..However,the extraction process for refined polysaccharides from Brassica rapa L.is slightly more complex and not suitable for mass production in factories.

关 键 词：芜菁多糖 分级醇沉 口服液 正交法 制备工艺 

分 类 号：TQ460.6[化学工程—制药化工]