宁夏地区部分食源性耐甲氧西林金黄色葡萄球菌分子分型、耐药特征及毒力基因分析  

Molecular typing,antibiotic resistance characteristics,and virulence gene analysis of some food-borne methicillin-resistant Staphylococcus aureus in Ningxia

作　　者：沈梅[1] 魏琼[1] 杨丽娟 刘翔[1] 马学旻[1] SHEN Mei;WEI Qiong;YANG Li-Juan;LIU Xiang;MA Xue-Min(Ningxia Hui Autonomous Region Center for Disease Control and Prevention,Ningxia Academy of Preventive Medicine Yinchuan 750004,China)

机构地区：[1]宁夏回族自治区疾病预防控制中心,宁夏预防医学科学院,银川750004

出　　处：《食品安全质量检测学报》2025年第5期262-268,共7页Journal of Food Safety and Quality

基　　金：宁夏自然科学基金项目(2022AAC03711)。

摘　　要：目的 了解宁夏地区部分食源性耐甲氧西林金黄色葡萄球菌(methicillin-resistant Staphylococcus aureu, MRSA)的分型特征和耐药特征以及与毒力基因携带情况。方法 收集宁夏地区部分食品风险监测分离的MRSA,分别进行药物敏感试验、脉冲场凝胶电泳(pulsed-fiedld gel electrophoresis, PFGE)以及全基因组测序分析。结果 9株来自食源性的MRSA菌株PFGE分型分为3簇8种型别其源性都在85%以上,全基因组测序结果 ST分型分为4种ST型,6株为ST59,其余3株分别为ST3355、ST7、ST965。MRSA均为多重耐药,携带耐药基因以及毒力基因也各不相同,导致的耐药表型也存在差异。结论 食源性分离MRSA基因组中存在大量与耐药和致病相关基因,这些基因给人类健康带来巨大威胁,因此持续监测并采取有效措施降低食品中MRSA的污染水平,以确保食品安全。Objective To investigate the type and drug resistance characteristics and virulence gene carrying of some food-borne methicillin-resistant Staphylococcus aureu(MRSA)in Ningxia.Methods MRSA isolates from some food risk monitoring in Ningxia were collected and subjected to drug susceptibility testing,pulsed-field gel electrophoresis(PFGE),and whole-genome sequencing analysis respectively.Results PFGE typing of 9 food-borne MRSA strains was divided into 3 clusters and 8 kinds of types,all of which were more than 85%provenance.ST typing of the whole genome sequencing results was divided into 4 kinds of ST types,6 strains were ST59,and the other 3 strains were ST3355,ST7 and ST965,respectively.MRSA were all multi-drug resistant,which carrying different resistance genes and virulence genes,resulting in different drug resistance phenotypes.Conclusion Foodborne MRSA isolates have a large number of genes related to antibiotic resistance and pathogenicity,which pose a significant threat to human health.Therefore,it is of great importance to continuously monitor and take effective measures to reduce the contamination level of MRSA in food to ensure food safety.

关 键 词：耐甲氧西林金黄色葡萄球菌 分型特征 耐药特征 毒力基因 

分 类 号：TS201.3[轻工技术与工程—食品科学]