基于生物酶辅助提取的多花黄精根茎成分及活性研究  

Study on Component and Activity of Polygonatum cyrtonema Hua by Enzyme-Assisted Extraction

作　　者：黄芳燕 麦迪乃·萨比尔 李悦 牛小敏 赖丹妮 刘京晶[1] 斯金平[1] 张新凤[1] HUANG Fangyan;MAIDINAI Sabier;LI Yue;NIU Xiaomin;LAI Danni;LIU Jingjing;SI Jinping;ZHANG Xinfeng(Zhejiang A&F University State Key Laboratory of Subtropical Silviculture,Hangzhou,Zhejiang 311300)

机构地区：[1]浙江农林大学省部共建亚热带森林培育国家重点实验室,浙江杭州311300

出　　处：《核农学报》2025年第4期773-783,共11页Journal of Nuclear Agricultural Sciences

基　　金：浙江省重大研发项目(2021C02020)。

摘　　要：为探讨酶水解对多花黄精(Polygonatum cyrtonema Hua)根茎的影响,本研究选取6种酶(纤维素酶、木瓜蛋白酶、果胶酶、β-葡聚糖酶、木聚糖酶和半纤维素酶)对多花黄精根茎进行水解,根据其总多糖、总皂苷和总酚含量以及酚类组成的变化确定复合酶组成,测定复合酶水解后多花黄精根茎醇提物的生物活性,并筛选主要差异物质进行相关性分析以探寻潜在活性成分。结果表明,木瓜蛋白酶、β-葡聚糖酶和木聚糖酶能够促进多花黄精根茎中酚类和皂苷类的高效释放,复合酶(木瓜蛋白酶∶β-葡聚糖酶∶木聚糖酶=3∶2∶1)水解可显著提高多花黄精根茎醇提物的抗氧化活性及α-葡萄糖苷酶抑制活性(P<0.001),筛选得到13种酚类物质、1种皂苷类物质与多花黄精根茎醇提物的降血糖活性密切相关,其含量在复合酶水解后显著增加(P<0.05)。本研究证实了酶水解对多花黄精根茎的成分释放及活性表现的改善效果,为黄精提取优化提供了方法参考。To investigate the impact of enzyme hydrolysis on Polygonatum cyrtonema Hua rhizome(PC),this study analyzed the effects of six enzymes(cellulase,papain,pectinase,β-dextranase,xylanase,and hemicellulase)on PC’s total polysaccharides,total saponins,total phenols,and phenolic composition to establish an optimal multi-enzyme combination.Subsequently,the biological activity of PC’s alcohol extract was measured,and correlation analysis was conducted with the main differential substances.The results showed that,papain,β-dextranase,and xylanase notably enhanced the release of phenols and saponins in PC.Furthermore,the antioxidant activity andα-glucosidase inhibitory activity of PC’s alcohol extract were significantly improved by the treatment with the multi-enzyme hydrolysis(papain∶β-dextranase∶xylanase=3∶2∶1,P<0.001).Thirteen phenols and one saponin were closely associated with theα-glucosidase inhibitory activity of PC and increased significantly following multi-enzyme hydrolysis(P<0.05).These findings confirmed that enzyme hydrolysis was an effective method to enhance the release of components and improve the biological activity of PC,which can provide a method reference for the optimization of PC’s extract.

关 键 词：多花黄精根茎 酶水解 成分 抗氧化活性 α-葡萄糖苷酶抑制活性 

分 类 号：R284.2[医药卫生—中药学]