补中益气方不同剂型中活性成分含量及其生物活性比较  

Comparison of active constituent contents and their biological activities of Buzhong Yiqi Recipe with different dosage forms

作　　者：贺嫣然 王婧 许家强 夏昭昭 刘应蛟 董子舒 明良山[1,2,3,4] 刘红宁 樊启猛[1,2,3,4] HE Yan-ran;WANG Jing;XU Jia-qiang;XIA Zhao-zhao;LIU Ying-jiao;DONG Zi-shu;MING Liang-shan;LIU Hong-ning;FAN Qi-meng(Advanced Research Institute,Jiangxi University of Chinese Medicine,Nanchang 330004,China;College of Pharmacy,Jiangxi University of Chinese Medicine,Nanchang 330004,China;Research Center for Differention and Development of Traditional Chinese Medicine Basic Theory,Jiangxi University of Chinese Medicine,Nanchang 330004,China;Jiangxi Provincial Key Laboratory for Traditional Chinese Medicine Etiopathogenisis,Nanchang 330004,China)

机构地区：[1]江西中医药大学高等研究院,江西南昌330004 [2]江西中医药大学药学院,江西南昌330004 [3]江西中医药大学中医基础理论分化发展研究中心,江西南昌330004 [4]江西省中医病因生物学重点实验室,江西南昌330004

出　　处：《中成药》2025年第2期357-364,共8页Chinese Traditional Patent Medicine

基　　金：中央本级重大增减支项目(2060302);国家自然科学基金项目(82360782);江西省自然科学基金项目(20232BAB206170);江西省教育厅科学技术研究项目(GJJ2400812);江西省中医药管理局科技计划项目(2021B617);江西中医药大学科研基金项目(2021BSZR010);江西中医药大学校级科技创新团队发展计划项目(CXTD22007)。

摘　　要：目的比较补中益气方大蜜丸、小蜜丸、水丸、浓缩丸、颗粒、合剂、汤剂中总糖、总蛋白、总酚、总黄酮、毛蕊异黄酮葡萄糖苷、甘草苷、党参炔苷、槲皮素、异阿魏酸、橙皮苷、甘草酸的含量及其抗氧化、降血糖活性。方法蒽醌-硫酸法测定总糖含量,考马斯亮蓝法测定总蛋白含量,福林酚比色法测定总酚含量,亚硝酸钠-硝酸铝法测定总黄酮含量,HPLC法测定7种指标成分含量,检测其对DPPH·自由基、ABTS+自由基、羟自由基的清除能力和还原力,以及对α-葡萄糖苷酶活性的抑制能力,再进行相关性分析。结果总糖、总蛋白、总酚、总黄酮含量在不同剂型之间存在显著差异(P<0.05,P<0.01)。汤剂中毛蕊异黄酮葡萄糖苷、甘草苷、党参炔苷、槲皮素含量最高,合剂中异阿魏酸、橙皮苷、甘草酸含量最高。水丸抗氧化活性最强,浓缩丸、合剂较弱;大蜜丸降血糖活性最强,汤剂最弱。总蛋白、总酚、总黄酮、甘草苷含量与抗氧化活性呈显著正相关(P<0.05,P<0.01),而橙皮苷含量与后者呈显著负相关(P<0.05);总蛋白、毛蕊异黄酮葡萄糖苷、党参炔苷、槲皮素含量与降血糖活性呈显著负相关(P<0.05,P<0.01)。结论补中益气方不同剂型活性成分含量及其生物活性存在差异,总糖、总蛋白、总黄酮、毛蕊异黄酮葡萄糖苷、甘草苷、橙皮苷、党参炔苷、槲皮素可作为该方质量控制指标。AIM To compare total sugar,total protein,total phenol,total flavonoid,calycosin-7-O-β-D-glucoside,liquiritin,lobetyolin,quercetin,isoferulic acid,hesperidin,glycyrrhizic acid contents and their antioxidant activities,hypoglycemic activities of big honey pill,small honey pill,water pill,concentrated pill,granule,mixture and decoction of Buzhong Yiqi Recipe.METHODS Anthraquinone-sulfuric acid method,Coomassie brilliant blue method,Folin-phenol colorimetry method,sodium nitrite-aluminum nitrate method and HPLC were adopted in the content determination of total sugar,total protein,total phenol,total flavonoid and seven constituents,respectively,after which the scavenging capacities,reducing powers on DPPH·free radical,ABTS+free radical,hydroxyl free radical,and inhibition capacity onα-glucosidase activity were detected.Subsequently,correlation analysis was performed.RESULTS Total sugar,total protein,total phenol and total flavonoid contents demonstrated significant differences among different dosage forms(P<0.05,P<0.01).Calycosin-7-O-β-D-glucoside,glycyrrhizin,codonoside and quercetin displayed the highest contents in the decoction,while those of isoferulic acid,hesperidin and glycyrrhizin were observable in the mixture.The water pill exhibited the strongest antioxidant activity,while those of the concentrated pill and mixture were weak;the big honey pill exhibited the strongest hypoglycemic activity,while that of the decoction was the weakest.Total protein,total phenol,total flavonoid and liquiritin contents displayed significant positive correlations between antioxidant activity(P<0.05,P<0.01),while hesperidin content displayed significant negative correlation between the latter(P<0.05);total protein,calycosin-7-O-β-D-glucoside,codonoside and quercetin contents displayed significant negative correlations between hypoglycemic activity(P<0.05,P<0.01).CONCLUSION Active constituent contents and their biological activities of Buzhong Yiqi Recipe with different dosage forms exist differences,total sugar,total protei

关 键 词：补中益气方 剂型 活性成分 含量测定 抗氧化活性 降血糖活性 

分 类 号：R927.2[医药卫生—药学]