Baitouweng decoction suppresses growth of esophageal carcinoma cells through miR-495-3p/BUB1/STAT3 axis  

作　　者：Hui Yang Xiao-Wei Chen Xue-Jie Song Hai-Yang Du Fu-Chun Si 

机构地区：[1]Henan Key Laboratory of Traditional Chinese Medicine Syndrome and Prescription in Signaling,Henan International Joint Laboratory of TCM Syndrome and Prescription in Signaling,Traditional Chinese Medicine School,Henan University of Chinese Medicine,Zhengzhou 450046,Henan Province,China

出　　处：《World Journal of Gastrointestinal Oncology》2024年第7期3193-3210,共18页世界胃肠肿瘤学杂志(英文)

基　　金：Supported by National Natural Science Foundation of China,No.81550014;Henan Provincial Science and Technology Research Project,No.222102310187 and No.242102310582;Henan Province Postdoctoral Research Project,No.202103092.

摘　　要：BACKGROUND Esophageal carcinoma(EC)is one of the most prevalent cancers in human populations worldwide.Baitouweng decoction is one of the most important Chinese medicine formulas,with the potential to treat cancer.AIM To investigate the role and mechanism of Baitouweng decoction on EC cells.METHODS Differentially expressed genes(DEGs)in EC tissues and normal tissues were screened by the cDNA microarray technique and by bioinformatics methods.The target genes of microRNAs were predicted based on the TargetScan database and verified by dual luciferase gene reporter assay.We used Baitouweng decoction to intervene EC cells,and detected the activity of EC9706 and KYSE150 cells by the MTT method.Cell cycle and apoptosis were measured by flow cytometry.The expression of BUB1 mRNA and miR-495-3p was measured by qRT-PCR.The protein levels of BUB1,STAT3,p-STAT3,CCNB1,CDK1,Bax,Caspase3,and Caspase9 were measured by Western blot analysis.The migration and invasion abilities of the cells were measured by wound-healing assay and Transwell invasion assay,respectively.RESULTS DEGs identified are involved in biological processes,signaling pathways,and network construction,which are mainly related to mitosis.BUB1 was the key hub gene,and it is also a target gene of miR-495-3p.Baitouweng decoction could upregulate miR-495-3p and inhibit BUB1 expression.In vitro experiments showed that Baitouweng decoction significantly inhibited the migration and invasion of EC cells and induced apoptosis and G2/M phase arrest.After treatment with Baitouweng decoction,the expression of Bax,Caspase 3,and Caspase 9 in EC cells increased significantly,while the expression of BUB1,CCNB1,and CDK1 decreased significantly.Moreover,the STAT3 signaling pathway may play an important role in this process.CONCLUSION Baitouweng decoction has a significant inhibitory effect on EC cell growth.BUB1 is a potential therapeutic target for EC.Further analysis showed that Baitouweng decoction may inhibit the growth of EC cells by upregulating miR-495-3p targeting the B

关 键 词：Baitouweng decoction Esophageal cancer miR-495-3p BUB1 STAT3 signaling pathway 

分 类 号：R73[医药卫生—肿瘤]