低聚糖与鼠李糖乳酪杆菌MP108协同调节大鼠免疫功能的研究  

Study on the synergistic regulation of immune function in rats by oligosaccharides and Lactobacillus rhamnosus MP108

作　　者：严浩东 王鹏 于俊娟 康文丽 潘丽娜 汪家琦 李意思 顾瑞霞[1,2] 张臣臣 YAN Haodong;WANG Peng;YU Junjuan;KANG Wenli;PAN Lina;WANG Jiaqi;LI Yisi;GU Ruixia;ZHANG Chenchen(College of Food Science and Engineering,Yangzhou University,Yangzhou 225000,China;Jiangsu Key Laboratory of Dairy Biotechnology and Safety Control,Yangzhou University,Yangzhou 225000,China;College of Public Health Administration,Jiangsu Health Vocational College,Nanjing 210029,China;College of Culinary Technology,Jiangsu College of Tourism,Yangzhou 225000,China;Ausnutria Dairy Corporation Ltd.,Changsha 410127,China;Hunan Engineering Research Center of Human Microecological Products,Changsha 410127,China)

机构地区：[1]扬州大学食品科学与工程学院,江苏扬州225000 [2]扬州大学,江苏省乳品生物技术与安全控制重点实验室,江苏扬州225000 [3]江苏卫生健康职业学院公共卫生管理学院,江苏南京210029 [4]江苏旅游职业学院烹饪科技学院,江苏扬州225000 [5]澳优乳业(中国)有限公司,湖南长沙410127 [6]人体微生态制品湖南省工程研究中心,湖南长沙410127

出　　处：《食品与发酵工业》2025年第5期134-139,共6页Food and Fermentation Industries

基　　金：湖南省科技创新计划资助项目(2022RC1063);扬州大学“青蓝工程”项目;扬州大学“高端人才支持计划”项目;扬州市-扬州大学市校合作共建科技创新平台项目(YZ2020265)。

摘　　要：鼠李糖乳酪杆菌MP108是一株可以用于婴幼儿食品的益生菌菌株,为了验证其与低聚糖在免疫调节功能上是否具有协同作用,使用40 mg/kg BW的环磷酰胺腹腔注射3 d的方式对Wistar大鼠进行造模,构建免疫低下模型。造模成功后使用低聚糖和鼠李糖乳酪杆菌MP108进行干预,并观察其相关生理指标的恢复效果。结果显示,MP108组的脾脏指数的调节效果最好,与作为阳性对照的药物无显著性差异(P>0.05),而2′-FL则有助于维持胸腺指数。在对分泌TNF-α能力的调节方面,2′-FL组、复配+MP108组和2′-FL+MP108组则与阳性对照组无显著性差异(P>0.05);在分泌IL-2能力上,除MP108组外,其他组均具有与阳性对照相似的调节效果。低聚糖与益生菌组合后对免疫球蛋白的分泌量影响较小。综上所述,鼠李糖乳酪杆菌MP108和低聚糖对免疫低下大鼠的各项指标的调节效果各有优势,呈现出协同作用。Lactobacillus rhamnosus MP108 is a probiotic strain that can be used in infant food.To verify whether it has a synergistic effect with oligosaccharides in immunomodulatory functions,an immunocompromised model was constructed using Wistar rats.The method was to use 40 mg/kg BW of cyclophosphamide injected intraperitoneally for three days after successful modeling,intervention was carried out using oligosaccharides and L.rhamnosus MP108.The recovery effect of their relevant physiological indexes was observed.The results showed that the MP108 group had the best regulation of splenic index,which was not significantly different from that of the drug used as a positive control(P>0.05),while 2′-FL helped to maintain thymic index.In terms of modulation of the ability to secrete TNF-α,the 2′-FL group,the compound+MP108 group and the 2′-FL+MP108 group,on the other hand,were not significantly different(P>0.05)from that of the positive control;In terms of IL-2 secretion ability,all groups except MP108 group had similar modulation effect as the positive control.The combination of oligosaccharides and probiotics had less effect on immunoglobulin secretion.In summary,L.rhamnosus MP108 and oligosaccharides have their own advantages in regulating the various indexes of immunocompromised rats,showing synergistic effects.

关 键 词：鼠李糖乳酪杆菌 低聚糖 益生作用 免疫调节 

分 类 号：TS201.3[轻工技术与工程—食品科学]